| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| 1g |
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| Other Sizes |
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| ln Vitro |
Atractylenolide III (1-100 μM, 48 h) stimulates A549 cells and induces caspase-3 and caspase-9 activation and PARP localization [1]. Atractylenolide III (1-100 μM, 72 hours) decreases the swelling of HUVECs and Atractylenolide III (1-100 μM) inhibits thymobasalpoietin (TSLP)-induced pro-inflammatory cytokines (IL-6, IL- 1b, TNF-α and IL- ).
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| ln Vivo |
Atractylenolide III (5 and 10 mg/kg, p.o.) decreases the 70% ethanol-induced gastrointestinal ulcer in rats and has gastroprotective properties[2]. Atractylenolide III (30 mg/kg, orally administered for 14 or 28 days) attenuates depressive and anxiogenic-like behaviors in rat depression models caused by chronic unpredictable mild stress (CUMS) and lipopolysaccharide (LPS) (HY-D1056)[5].
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| Cell Assay |
Western Blot Analysis[1]
Cell Types: A549 Tested Concentrations: 1, 10, 100 μM Incubation Duration: 24 h, 48 h Experimental Results: Increased active forms of caspase-9, caspase-3 and caspase-3. PARP was cleaved at 48 hrs (hours). The expression of pro-apoptotic protein bax increased at 24 hrs (hours) and induced AIF translocation to the nucleus. |
| Animal Protocol |
Animal/Disease Models: 70% ethanol induced gastric ulcer in rats [2]
Doses: 5 and 10 mg/kg Route of Administration: Oral Experimental Results:Inhibition of gastric ulcer Formation and gastric mucosal necrosis and erosion. Downregulates MMP-2/9 expression through activation of TIMP-2 and TIMP-1 expression. |
| References |
[1]. Kang TH, et al. Atractylenolide III, a sesquiterpenoid, induces apoptosis in human lung carcinoma A549 cells via mitochondria-mediated death pathway. Food Chem Toxicol. 2011 Feb;49(2):514-9.
[2]. Wang KT, et al. Gastroprotective activity of atractylenolide III from Atractylodes ovata on ethanol-induced gastric ulcer in vitro and in vivo. J Pharm Pharmacol. 2010 Mar;62(3):381-8. [3]. Kim HK, et al. Toxicity of atractylon and atractylenolide III Identified in Atractylodes ovata rhizome to Dermatophagoides farinae and Dermatophagoides pteronyssinus. J Agric Food Chem. 2007 Jul 25;55(15):6027-31. [4]. Yoou MS, et al. Ameliorative effect of atractylenolide III in the mast cell proliferation induced by TSLP. Food Chem Toxicol. 2017 Aug;106(Pt A):78-85. [5]. Zhou Y, et al. Atractylenolide III reduces depressive- and anxiogenic-like behaviors in rat depression models. Neurosci Lett. 2021 Aug 10;759:136050. |
| Molecular Formula |
C15H20O3
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|---|---|
| Molecular Weight |
248.3175
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| Exact Mass |
248.14124450
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| CAS # |
73030-71-4
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| Related CAS # |
73030-71-4
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| Appearance |
Solid
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| SMILES |
CC1=C2C[C@H]3C(=C)CCC[C@@]3(C[C@@]2(OC1=O)O)C
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| InChi Key |
FBMORZZOJSDNRQ-GLQYFDAESA-N
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| InChi Code |
InChI=1S/C15H20O3/c1-9-5-4-6-14(3)8-15(17)12(7-11(9)14)10(2)13(16)18-15/h11,17H,1,4-8H2,2-3H3/t11-,14+,15-/m0/s1
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| Chemical Name |
(4aS,8aR,9aS)-9a-hydroxy-3,8a-dimethyl-5-methylidene-4,4a,6,7,8,9-hexahydrobenzo[f][1]benzofuran-2-one
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| Synonyms |
ICodonolactone; Atractylenolide III; 8β-Hydroxyasterolide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~50 mg/mL (~201.4 mM)
Ethanol: ~50 mg/mL (~201.4 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (10.07 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (10.07 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (10.07 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.0271 mL | 20.1353 mL | 40.2706 mL | |
| 5 mM | 0.8054 mL | 4.0271 mL | 8.0541 mL | |
| 10 mM | 0.4027 mL | 2.0135 mL | 4.0271 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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