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10mg |
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25mg |
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50mg |
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100mg |
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250mg |
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Aurantio-obtusin is a novel and potent anthraquinone isolated from Semen Cassiae. Aurantio-obtusin has a variety of biological effects, including those that are anti-oxidative, anti-coagulant, anti-inflammatory, and anti-hypertensive. Additionally, aurantio-obtusin, which relaxes systemic arteries in rats via an endothelial PI3K/AKT/eNOS-dependent signaling pathway, is a potential vasodilator. It is possible to treat diseases associated with allergies by using aurantio-obtusin, an inhibitor of allergic responses in IgE-mediated mast cells and anaphylactic models.
ln Vitro |
Aurantio-obtusin (6.25-100 μM; 24 h) can significantly reduce the production of NO and PGE2, and significantly inhibit IL-6, TNF-α and COX in RAW264.7 cells treated with LPS (0.2 μg/mL) -2 Aurantio-obtusin (6.25-100 μM; 12 h) inhibits NF-κB activation in RAW264.7 cells treated with LPS (0.2 μg/mL) by inhibiting i-κB and IKK phosphorylation. Aurantioobtusin (1-10000 nM) produces MA small resistance vasodilation in a concentration-dependent manner [1].
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ln Vivo |
In HFSW-induced mice, aurantio-obtusin (5–15 mg/kg; face; single dose) decreases lipid droplet accumulation and widespread steatosis in a dosage-dependent manner [3]. Oryza obtusina (5).
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Animal Protocol |
Animal/Disease Models: C57BL/6J mouse model [3]
Doses: 5 mg/ -15 mg/kg; receptor; single dose) Inhibits factor synthesis in HFSW-induced cytokines and promotes FAO activation of AMPK signaling and autophagy [3 ]. kg, 10 mg/kg, 15 mg/kg Route of Administration: po (oral gavage) on HFSW diet for 4 weeks, followed by varying doses of Aurantio-obtusin for a further 4 weeks. Experimental Results: HSFW can reduce the levels of TG, TC in liver and TG, ALT and AST in serum. Reduces the number and size of fat droplets in liver cells. AMPK phosphorylation was Dramatically increased in HFSW-induced mice. |
References |
[1]. Li, Shuzhen, et al. Aurantio-obtusin relaxes systemic arteries through endothelial PI3K/AKT/eNOS-dependent signaling pathway in rats. Journal of pharmacological sciences vol. 128,3 (2015): 108-15.
[2]. Hou Jingyi, et al. Anti-Inflammatory Effects of Aurantio-Obtusin from Seed of Cassia obtusifolia L. through Modulation of the NF-κB Pathway. Molecules (Basel, Switzerland) vol. 23,12 3093. 27 Nov. 2018. [3]. Zhou Fei, et al. Aurantio-Obtusin Attenuates Non-Alcoholic Fatty Liver Disease Through AMPK-Mediated Autophagy and Fatty Acid Oxidation Pathways. Frontiers in pharmacology vol. 12 826628. 11 Jan. 2022. |
Molecular Formula |
C17H14O7
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Molecular Weight |
330.2889
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Exact Mass |
330.074
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Elemental Analysis |
C, 61.82; H, 4.27; O, 33.91
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CAS # |
67979-25-3
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Related CAS # |
67979-25-3
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Appearance |
A crystalline solid
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SMILES |
CC1=CC2=C(C(=C1O)OC)C(=O)C3=C(C(=C(C=C3C2=O)O)OC)O
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InChi Key |
RNXZPKOEJUFJON-UHFFFAOYSA-N
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InChi Code |
InChI=1S/C17H14O7/c1-6-4-7-11(17(24-3)12(6)19)14(21)10-8(13(7)20)5-9(18)16(23-2)15(10)22/h4-5,18-19,22H,1-3H3
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Chemical Name |
1,3,7-trihydroxy-2,8-dimethoxy-6-methylanthracene-9,10-dione
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Synonyms |
Aurantio obtusin; Aurantio-obtusin; Aurantioobtusin
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO: 25~66 mg/mL (75.7~199.8 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (6.30 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.0276 mL | 15.1382 mL | 30.2764 mL | |
5 mM | 0.6055 mL | 3.0276 mL | 6.0553 mL | |
10 mM | 0.3028 mL | 1.5138 mL | 3.0276 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Effects of aurantio-obtusin on the cell viability of RAW264.7 cells. Molecules. 2018 Nov 27;23(12):3093. td> |
Figure 4. Effect of aurantio-obtusin on IL-6, TNF-α, PGE2 production and COX-2 protein expression in LPS-treated RAW264.7 cells. Molecules. 2018 Nov 27;23(12):3093. td> |
Fig. 5. Effect of Aurantio-obtusin on the phosphorylation and protein of eNOS in BAECs. J Pharmacol Sci. 2015 Jul;128(3):108-15. td> |