Size | Price | Stock | Qty |
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1mg |
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5mg |
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10mg |
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50mg |
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Other Sizes |
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Purity: ≥98%
BCI hydrochloride [(E)-BCI] is a novel, potent and allosteric inhibitor of Dusp6 (dual specificity phosphatase), which acts within the phosphatase domain to prevent the catalytic stimulation of phosphatase activity induced by ERK2 substrate binding. It inhibits DUSP6 and DUSP1 with EC50s of 13.3 and 8.0 μM in cells, respectively.
ln Vitro |
In RAW264.7 macrophages, BCI hydrochloride (100 ng/mL; 24 h) suppresses DUSP6 expression [2]. Lipopolysaccharide (LPS)-activated macrophages are inhibited in their expression by BCI hydrochloride (0-1 nM; 24 h). On the other hand, BCI hydrochloride (0-4 nM; 24 h) decreases ROS production and stimulates Nrf2 staining, which activates phagocytosis in LPS-activated macrophages[2].
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Cell Assay |
Western Blot Analysis[2]
Cell Types: RAW264.7 Macrophage Tested Concentrations: 100 ng/mL Incubation Duration: 24 hrs (hours) Experimental Results: demonstrated downregulation of DUSP6 protein. RT-PCR[2] Cell Types: RAW264.7 Macrophage Tested Concentrations: 0-1 nM Incubation Duration: 24 hrs (hours) Experimental Results: Inhibits the expression of IL-1β and IL-6 mRNA in LPS-activated macrophages. |
References |
[1]. Korotchenko VN, et al. In vivo structure-activity relationship studies support allosteric targeting of a dual specificity phosphatase. Chembiochem. 2014 Jul 7;15(10):1436-45.
[2]. Zhang F, et al. DUSP6 Inhibitor (E/Z)-BCI Hydrochloride Attenuates Lipopolysaccharide-Induced Inflammatory Responses in Murine Macrophage Cells via Activating the Nrf2 Signaling Axis and Inhibiting the NF-κB Pathway. Inflammation. 2019 Apr;42(2):672-681. |
Molecular Formula |
C22H24CLNO
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Molecular Weight |
353.88506
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CAS # |
95130-23-7
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Related CAS # |
BCI;1245792-51-1;(E/Z)-BCI;15982-84-0
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
O=C1/C(C(NC2CCCCC2)C3=C1C=CC=C3)=C\C4=CC=CC=C4.[H]Cl
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InChi Key |
JPATUDRDKCLPTI-CRDKNBMZSA-N
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InChi Code |
InChI=1S/C22H23NO.ClH/c24-22-19-14-8-7-13-18(19)21(23-17-11-5-2-6-12-17)20(22)15-16-9-3-1-4-10-16/h1,3-4,7-10,13-15,17,21,23H,2,5-6,11-12H21H/b20-15-
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Chemical Name |
2-Benzylidene-3-(cyclohexylamino)-2,3-dihydro-1H-inden-1-one hydrochloride
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Synonyms |
BCI hydrochloride
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~15.62 mg/mL (~44.14 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.56 mg/mL (4.41 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 15.6 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.56 mg/mL (4.41 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 15.6 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 1.56 mg/mL (4.41 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.8257 mL | 14.1287 mL | 28.2574 mL | |
5 mM | 0.5651 mL | 2.8257 mL | 5.6515 mL | |
10 mM | 0.2826 mL | 1.4129 mL | 2.8257 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.