Size | Price | Stock | Qty |
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5mg |
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10mg |
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Other Sizes |
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ln Vitro |
Bergamot is a competitive inhibitor of CYP1A1. Bergamotin inhibits CYP1A1, CYP1A2, CYP2B1 and CYP2B2, with IC50 of 0.192±0.029 μM, 5.077±0.31 μM, 9.495±0.979 μM, and 4.535±0.092 μM correspondingly [1]. Bergamot has powerful antiproliferative effects on A549 cells. Bergamot displayed concentration- and time-dependent growth inhibitory effects on these cells. Bergamot also suppresses the clonogenic activity of A549 cancer cells by lowering the amount of cancer colony-forming cells. Bergamot concentration-dependence also leads to a reduction in clonogenicity [2].
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ln Vivo |
A total of 20 female BALB/c nude mice were employed to assess the anticancer efficaciousness of bergamot in vivo. Mice were given injections of 1×106 cells/mouse of non-small cell lung carcinoma A549, which caused tumors. Mice were slaughtered when the tumors formed, the tumors were excised, and the volume and weight of the tumors were determined. According to the findings, the tumor weight decreased from 1.61 g in the PBS-treated group (control group) to 1.21, 0.42, and 0.15 g, respectively, after injections of 25, 50, and 100 mg/kg of bergamot. The tumor weight of nude mice in the bergamot group with the highest dose (100 mg/kg body weight) dropped more considerably (P<0.05) than the vehicle group. Similarly, the tumor volume in the PBS-treated group (control group) was decreased from 2.2 cm3 to 1.71, 1.1, and 0.51 cm3 by injections of 25, 50, and 100 mg/kg bergamot. Frequent measurements of the transplanted tumor volume revealed that the tumor volume of nude mice in the highest dose bergamot group (100 mg/kg body weight) was significantly (P<0.05) lower than that of the vehicle group [2].
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References |
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Additional Infomation |
Bergomottin is a furanocoumarin. It has a role as a metabolite.
Bergamottin has been reported in Angelica dahurica var. formosana, Citrus hassaku, and other organisms with data available. See also: Lime (Citrus) (part of). |
Molecular Formula |
C21H22O4
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Molecular Weight |
338.3970
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Exact Mass |
338.151
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CAS # |
7380-40-7
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PubChem CID |
5471349
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Appearance |
White to off-white solid powder
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Density |
1.2±0.1 g/cm3
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Boiling Point |
503.7±50.0 °C at 760 mmHg
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Melting Point |
75-80ºC
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Flash Point |
258.4±30.1 °C
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Vapour Pressure |
0.0±1.3 mmHg at 25°C
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Index of Refraction |
1.583
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LogP |
5.92
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Hydrogen Bond Donor Count |
0
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Hydrogen Bond Acceptor Count |
4
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Rotatable Bond Count |
6
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Heavy Atom Count |
25
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Complexity |
570
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Defined Atom Stereocenter Count |
0
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SMILES |
CC(=CCC/C(=C/COC1=C2C=CC(=O)OC2=CC3=C1C=CO3)/C)C
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InChi Key |
DBMJZOMNXBSRED-OQLLNIDSSA-N
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InChi Code |
InChI=1S/C21H22O4/c1-14(2)5-4-6-15(3)9-11-24-21-16-7-8-20(22)25-19(16)13-18-17(21)10-12-23-18/h5,7-10,12-13H,4,6,11H2,1-3H3/b15-9+
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Chemical Name |
4-[(2E)-3,7-dimethylocta-2,6-dienoxy]furo[3,2-g]chromen-7-one
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~50 mg/mL (~147.75 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (7.39 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (7.39 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.9551 mL | 14.7754 mL | 29.5508 mL | |
5 mM | 0.5910 mL | 2.9551 mL | 5.9102 mL | |
10 mM | 0.2955 mL | 1.4775 mL | 2.9551 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.