| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
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| ln Vitro |
Human hepatocellular carcinoma (HCC) is cytotoxic to BI-6015 (1.25–20 μM; 24-72 h) [1]. In HepG2 cells, BI-6015 (2.5-10 μM; 5-48 h) inhibits the HNF4α gene. Primary mouse hepatocytes are exposed to BI-6015 (5 μM; 3 d), which causes hepatic steatosis [1].
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| ln Vivo |
BI-6015 senses investigate HNF4α expression and hepatic steatosis; it is given intraperitoneally once day for five days in a row at a dose of 10–30 mg/kg [1]. In a human hepatocellular carcinoma model, BI-6015 (10-30 mg/kg; ip daily or once daily for 20-57 days) carries out cell sterilization [1].
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| Cell Assay |
Cell viability assay [1]
Cell Types: Hep3B-Luc cells and primary hepatocytes Tested Concentrations: 1.25, expression [1]. 2.5, 5, 10, 20 μM Incubation Duration: 24, 48, 72 hrs (hours) Experimental Results: Obvious toxicity to Hep3B cells, but does not affect primary hepatocytes. |
| References |
[1]. Kiselyuk A, et, al. HNF4α antagonists discovered by a high-throughput screen for modulators of the human insulin promoter. Chem Biol. 2012 Jul 27;19(7):806-18.
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| Molecular Formula |
C15H13N3O4S
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|---|---|
| Molecular Weight |
331.35
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| Exact Mass |
331.062
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| Elemental Analysis |
C, 54.37; H, 3.95; N, 12.68; O, 19.31; S, 9.68
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| CAS # |
93987-29-2
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| PubChem CID |
3269393
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| Appearance |
Solid powder
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| Density |
1.5±0.1 g/cm3
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| Boiling Point |
570.6±60.0 °C at 760 mmHg
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| Flash Point |
298.9±32.9 °C
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| Vapour Pressure |
0.0±1.6 mmHg at 25°C
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| Index of Refraction |
1.679
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| LogP |
3.53
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
23
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| Complexity |
556
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[O-][N+](C1C=C(S(N2C(C)=NC3C2=CC=CC=3)(=O)=O)C(C)=CC=1)=O
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| InChi Key |
ILVCPQPMRPHZSG-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C15H13N3O4S/c1-10-7-8-12(18(19)20)9-15(10)23(21,22)17-11(2)16-13-5-3-4-6-14(13)17/h3-9H,1-2H3
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| Chemical Name |
2-methyl-1-(2-methyl-5-nitrophenyl)sulfonylbenzimidazole
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| Synonyms |
BI6015 BI-6015 BI 6015
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~150.90 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (7.54 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.0180 mL | 15.0898 mL | 30.1796 mL | |
| 5 mM | 0.6036 mL | 3.0180 mL | 6.0359 mL | |
| 10 mM | 0.3018 mL | 1.5090 mL | 3.0180 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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