| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| 1g |
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Purity: ≥98%
Bisbenzimide, formerly known as Pibenzimol, Hoechst 33258, is a cell-permeable, benzimidazole dye that binds to the minor groove of double stranded DNA with preference for adenine and thymine-rich sequences.
| ln Vitro |
1.1 Hoechst working solution preparation: 10 milligrams should dissolve in 5 mL DMSO. It is advised to store the stock solution in the dark at 4°C or -20°C to prevent recurrent freezing and thawing. 1.2 Hoechst stock solution preparation A Hoechst working solution with a final concentration of 10 μg/mL is created by dissolving the stock solution in either PBS or serum-free cell culture medium. Note: Please modify the Hoechst working fluid concentration based on the current circumstances. 1. Staining of cells 2.1 Suspension plates with six wells a. Discard the supernatant after centrifuging at 1000g for three to five minutes at 4°C. Use PBS to wash twice, for five minutes each time. The density of cells is 1×106/mL. c. After adding 1 mL of the working solution, wait three to ten minutes. c. Centrifuge for 3–4 minutes at 400 g and 4°C, discarding the supernatant. d. Use PBS to wash twice, for five minutes each time. e. Re-suspend the cells in PBS or serum-free culture media. observation using a flow cytometer or a fluorescence microscope 2.2 Cells that adhere On sterile coverslips, cultivate adherent cells (a). b. Aspirate extra cells and remove the coverslip from the culture medium. b. Pour in 100 μL of working solution, then give it a little shake to fully cover. Prelude: 3–10 minutes for cells. d. Wash your heart twice, for five minutes each time. Cursor matter or observation using a flow cytometer. Note 1: Please modify the Hoechst working fluid concentration based on the current circumstances. 2. This product is intended only for use in research and development; it cannot be used for home, medical, or other applications. 3. Please wear a lab coat and immediate gloves when operating for your own health and safety.
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| References |
[1]. Wang XJ, et al. Newly synthesized bis-benzimidazole derivatives exerting anti-tumor activity through inductionof apoptosis and autophagy. Bioorg Med Chem Lett. 2012 Oct 1;22(19):6297-300.
[2]. Stolić I, et al. Synthesis, DNA/RNA affinity and antitumour activity of new aromatic diamidines linked by 3,4-ethylenedioxythiophene. Eur J Med Chem. 2011 Feb;46(2):743-5 |
| Molecular Formula |
C25H27CL3N6O
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|---|---|
| Molecular Weight |
533.882
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| CAS # |
23491-45-4
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| Related CAS # |
Hoechst 33258;23491-44-3
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| Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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| SMILES |
InChI=1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29)3*1H
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| InChi Key |
4-[6-(4-methyl-1-piperazinyl)[2,6'-bi-1H-benzimidazol]-2'-yl]-phenol, trihydrochloride
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| InChi Code |
SMNPLAKEGAEPJD-UHFFFAOYSA-N
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| Chemical Name |
Bisbenzimide Bisbenzimide trihydrochloride Pibenzimol Pibenzimol HCl Pibenzimol trihydrochloride. H 33258 HOE 33258 Hoechst 33258 NSC 322921.
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~187.31 mM)
H2O : ~12.5 mg/mL (~23.41 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: 9.09 mg/mL (17.03 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication (<60°C).
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.8731 mL | 9.3654 mL | 18.7308 mL | |
| 5 mM | 0.3746 mL | 1.8731 mL | 3.7462 mL | |
| 10 mM | 0.1873 mL | 0.9365 mL | 1.8731 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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