| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
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| ln Vitro |
Guidelines for usage 1. BODIPY493/503 methyl bromide working solution composition 1.1 Prepare the stock solution by adding 1 milligram of BODIPY493/503 methyl bromide to 10 mM of anhydrous DMSO (382 μL). Note: To generate a 1-10 μM BODIPY493/503 methyl bromide working solution, it is advised to aliquot the BODIPY493/503 methyl bromide storage solution into the 1.2 working solution rather than using pre-prepared serum-free cell culture medium or PBS. Please prepare the BODIPY493/503 methyl bromide working solution for usage by adjusting its concentration to the current circumstances. 2. Suspended cell staining (2.1): Centrifuge cells, add PBS, then wash twice for five minutes each time. 2.2 Add 1 mL of BODIPY493/503 methyl bromide working solution and stir for 5–30 minutes when the cell density is 1×106/mL. 2.3 Centrifuge at 400g for 3–4 minutes and discard the supernatant. 2.4 After adding PBS, wash the cells twice for five minutes each. 2.5 Re-suspend the cells in 1 milliliter of PBS or serum-free media, and use a flow cytometer or fluorescence microscope to observe. 3. Adherent cell staining: 3.1 Grow the adherent cells on sterile coverslips. 3.2 Cover the cells fully with 100 μL of the dye working solution from step 3.3, give them a gentle shake, and sterilize for a duration of 5 to 30 minutes. 3.4 Aspirate the dye working solution, rinse with culture medium two or three times for five minutes each, and use a flow cytometer or fluorescence microscope to monitor. Conditions for storage: Keep for a year at -20°C and out of direct sunlight. Observations 1. Kindly modify the BODIPY493/503 methyl bromide working solution concentration and the left-hand time interval based on the current circumstances. 2. It is advised that the experiment include an acute control. Before doing any further tests, the cells need to be treated to a 30 μM oleic acid carrier for 8 hours. 3. This product is not intended for use in clinical diagnosis or treatment; rather, it should only be used by experts conducting scientific research. 4. When working, please wear immediate gloves and a lab coat for your health and safety.
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| References |
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| Molecular Formula |
C14H16BN2F2BR
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|---|---|
| Molecular Weight |
341.00204
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| Exact Mass |
340.055
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| CAS # |
216434-81-0
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| PubChem CID |
71774308
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| Appearance |
Brown to reddish brown solid powder
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| LogP |
3.201
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
20
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| Complexity |
525
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
JLSHPOSUMKVMAP-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C14H16BBrF2N2/c1-8-5-10(3)19-13(8)12(7-16)14-9(2)6-11(4)20(14)15(19,17)18/h5-6H,7H2,1-4H3
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| Chemical Name |
8-(bromomethyl)-2,2-difluoro-4,6,10,12-tetramethyl-3-aza-1-azonia-2-boranuidatricyclo[7.3.0.03,7]dodeca-1(12),4,6,8,10-pentaene
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~10 mg/mL (~29.33 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 1 mg/mL (2.93 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9326 mL | 14.6628 mL | 29.3255 mL | |
| 5 mM | 0.5865 mL | 2.9326 mL | 5.8651 mL | |
| 10 mM | 0.2933 mL | 1.4663 mL | 2.9326 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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