Size | Price | Stock | Qty |
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2g |
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5g |
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10g |
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Other Sizes |
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Purity: ≥98%
BTS is a novel and potent inhibitor of ATPase activity of skeletal muscle myosin II subfragment 1 (S1), specifically the Ca2+-stimulated myosin S1 ATPase (IC50 = 5 μM).
ln Vitro |
BTS (2–12 μM) suppresses Ca2+-stimulated myosin S1 ATPase activity in the absence of actin, with an IC50 of about 5 μM [1]. The heavy myosin (HMM) sliding rate is reversibly inhibited by BTS (2–20 μM) [1]. In the presence of ADP, BTS (100 μM) releases myosin from actin [1]. With an IC50 of roughly 3 μM and 1 μM, respectively, BTS (0-20 μM) reversibly reduces Ca2+-activated tension in medium-length skinned skeletal muscle fibers of rabbit and frog [1].
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References |
[1]. Cheung A, et al. A small-molecule inhibitor of skeletal muscle myosin II. Nat Cell Biol. 2002 Jan;4(1):83-8.
[2]. Shaw MA, et, al. Mechanism of inhibition of skeletal muscle actomyosin by N-benzyl-p-toluenesulfonamide. Biochemistry. 2003 May 27;42(20):6128-35. |
Molecular Formula |
C14H15NO2S
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Molecular Weight |
261.33
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Exact Mass |
261.0823
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CAS # |
1576-37-0
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
S(C1C([H])=C([H])C(C([H])([H])[H])=C([H])C=1[H])(N([H])C([H])([H])C1C([H])=C([H])C([H])=C([H])C=1[H])(=O)=O
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Synonyms |
BnNHTs BTS
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ≥ 33.33 mg/mL (~127.54 mM)
H2O : ~0.1 mg/mL (~0.38 mM) |
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (9.57 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.57 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (9.57 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.8266 mL | 19.1329 mL | 38.2658 mL | |
5 mM | 0.7653 mL | 3.8266 mL | 7.6532 mL | |
10 mM | 0.3827 mL | 1.9133 mL | 3.8266 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.