Size | Price | Stock | Qty |
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5mg |
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10mg |
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25mg |
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50mg |
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100mg |
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250mg |
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Other Sizes |
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ln Vivo |
BU224 hydrochloride (5 mg/kg, intraperitoneal injection, twice daily for 10 days) can improve behavioral performance and memory function in 5XFAD mice [1]. BU224 hydrochloride (5 mg/kg, IP, twice daily for 10 days) decreases the levels of the microglia marker Iba1 and the proinflammatory cytokines IL-1β and TNF-α in 5XFAD animals and enhances the Expression of glial cell marker GFAP[1]. BU224 hydrochloride (10 mg/kg, intraperitoneal injection, once) lowers the immobility of rats in the FST (forced swim test), demonstrating antidepressant-like effects [2].
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Animal Protocol |
Animal/Disease Models: 5XFAD mice and WT littermates (6 months old) [1]
Doses: 0 mg/kg, 5 mg/kg Route of Administration: IP twice (two times) daily for 10 days Experimental Results: Significant increase immobility (54% increase in freezing) and reversed memory deficits secondary to Aβ deposition in the brain. |
References |
[1]. Mirzaei N, et al. Imidazoline ligand BU224 reverses cognitive deficits, reduces microgliosis and enhances synaptic connectivity in a mouse model of Alzheimer's disease. Br J Pharmacol. 2021 Feb;178(3):654-671.
[2]. Finn DP, et al. Behavioral, neuroendocrine and neurochemical effects of the imidazoline I2 receptor selective ligand BU224 in naive rats and rats exposed to the stress of the forced swim test. Psychopharmacology (Berl). 2003 May;167(2):195-202. [3]. Qiu Y, et al. Discriminative stimulus effects of the imidazoline I2 receptor ligands BU224 and phenyzoline in rats. Eur J Pharmacol. 2015 Feb 15;749:133-41. |
Molecular Formula |
C12H12CLN3
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Molecular Weight |
233.699
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Elemental Analysis |
C, 61.67; H, 5.18; Cl, 15.17; N, 17.98
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CAS # |
205437-64-5
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Related CAS # |
205437-64-5 (HCl);187173-05-3;
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Appearance |
Solid powder
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SMILES |
Cl.N1CCN=C1C1C=CC2C(=CC=CC=2)N=1
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Synonyms |
BU-224 HCl; BU-224; BU 224; BU224;
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~12.5 mg/mL (~53.49 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (5.35 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (5.35 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 4.2790 mL | 21.3950 mL | 42.7899 mL | |
5 mM | 0.8558 mL | 4.2790 mL | 8.5580 mL | |
10 mM | 0.4279 mL | 2.1395 mL | 4.2790 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.