| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vitro |
On lipopolysaccharide-stimulated C8-B4 cells, cefazolin sodium pentahydrate (0-300 μg/ml; 6 or 24 hours) exhibits a direct anti-inflammatory action [2]. Cefazolin sodium pentahydrate (0-400 μM; 72 hours) treatment suppresses IL-2, IL-4, and IL-15-induced cell growth [3]. The 30 minute administration of cefazolin sodium pentahydrate (0-400 μM) suppresses the phosphorylation of JAK3 induced by IL-2, IL-4, IL-15, and IL-21 [3].
|
|---|---|
| ln Vivo |
Cefazolin sodium pentahydrate (subcutaneous injection; 300-500 mg/kg; once daily; 5 d) medication can improve the postoperative learning and memory capacity of rats [2].
|
| Cell Assay |
Cell Viability Assay[2]
Cell Types: C8-B4 Cell Tested Concentrations: 0, 50, 100, 150, 200, 250 or 300 μg/ml Incubation Duration: 6 or 24 hrs (hours) Experimental Results: Complete inhibition of IL-1β with increasing doses, but The increase in IL-6 was inhibited only at 200 μg/ml. Cell proliferation assay[3] Cell Types: PBMC and TF-1 Cell Tested Concentrations: 0, 100, 200 and 400 μM Incubation Duration: 72 hrs (hours) Experimental Results: diminished cell proliferation induced by IL-2, IL-4 and IL-15, indicating Cefazolin not only interferes with IL-15Rα but also with IL-2/IL-15Rβ and/or γc. Western Blot Analysis [3] Cell Types: PBMC, NK-92 and TF-1 Cell Tested Concentrations: 0, 100, 200 and 400 μM Incubation Duration: 30 minutes Experimental Results: The phosphorylation of JAK3 was diminished after cytokine treatment, concluding that the inhibition of γc Receptor signal transduction. |
| Animal Protocol |
Animal/Disease Models: 6 to 8 weeks old male CD-1 mice underwent clinical exploratory laparotomy [2]
Doses: 300-500 mg/kg Route of Administration: subcutaneous injection; 300-500 mg/kg; one time/day; 5-day Experimental Results: Surgery-induced learning and memory dysfunction is attenuated. |
| References | |
| Additional Infomation |
Cefazolin sodium hydrate is a cephalosporin.
|
| Molecular Formula |
C14H13N8O4S3-.NA+.5[H2O]
|
|---|---|
| Molecular Weight |
566.56542
|
| Exact Mass |
566.065
|
| CAS # |
115850-11-8
|
| Related CAS # |
Cefazolin sodium;27164-46-1;Cefazolin;25953-19-9
|
| PubChem CID |
23678598
|
| Appearance |
Typically exists as solid at room temperature
|
| Hydrogen Bond Donor Count |
6
|
| Hydrogen Bond Acceptor Count |
17
|
| Rotatable Bond Count |
7
|
| Heavy Atom Count |
35
|
| Complexity |
746
|
| Defined Atom Stereocenter Count |
2
|
| SMILES |
CC1=NN=C(S1)SCC2=C(N3C([C@@H](NC(CN4C=NN=N4)=O)[C@H]3SC2)=O)C(O)=O.[Na].O.O.O.O.O.[H]
|
| InChi Key |
KHQJKXHCWOHDQD-HGUWTHONSA-M
|
| InChi Code |
InChI=1S/C14H14N8O4S3.Na.5H2O/c1-6-17-18-14(29-6)28-4-7-3-27-12-9(11(24)22(12)10(7)13(25)26)16-8(23)2-21-5-15-19-20-21;;;;;;/h5,9,12H,2-4H2,1H3,(H,16,23)(H,25,26);;5*1H2/q;+1;;;;;/p-1/t9-,12-;;;;;;/m1....../s1
|
| Chemical Name |
sodium;(6R,7R)-3-[(5-methyl-1,3,4-thiadiazol-2-yl)sulfanylmethyl]-8-oxo-7-[[2-(tetrazol-1-yl)acetyl]amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate;pentahydrate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.7650 mL | 8.8250 mL | 17.6501 mL | |
| 5 mM | 0.3530 mL | 1.7650 mL | 3.5300 mL | |
| 10 mM | 0.1765 mL | 0.8825 mL | 1.7650 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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