| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
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| ln Vitro |
Ceranib-2 treatment (10 nM-10 µM; 72 hours; SKOV3 cells) reduces the growth and/or survival of cells, with an IC50 value of 0.73 µM [1]. After 48 hours of treatment with ceretib-2 (0.75-1.5 µM; SKOV3 cells), cells accumulate in the sub-G1 (apoptotic), G2, and S (0.75 µM only) phases of the cell cycle, resulting in a drop in total cell quantity. G1 phase cells [1]. Ceranib-2 inhibits ceramidase activity in a dose-dependent manner, causing a 50% drop at 28 μM. It also induces the buildup of various ceramide species and lowers levels of sphingosine and S1P [1].
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| ln Vivo |
Treatment with Ceranib-2 (20–50 mg/kg; i.p.; once daily, five days a week; for three weeks; female Balb/c mice) in syngeneic tumor models slows the growth of tumors without hematologic suppression or overt toxicity [1]. After administering 50 mg/kg of Ceranib-2 intraperitoneally, the levels gradually increased and at the 2-hour mark, the peak plasma concentration was approximately 40 μM. Ceranib-2's half-life of less than two hours suggests that it is removed [1].
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| Cell Assay |
Cell Proliferation Assay[1]
Cell Types: SKOV3 Cell Tested Concentrations: 10 nM-10 µM Incubation Duration: 72 hrs (hours) Experimental Results: Ceranib-2 inhibits cell proliferation and/or survival with an IC50 value of 0.73 µM. Cell cycle analysis[1] Cell Types: SKOV3 Cell Tested Concentrations: 0.75 μM or 1.5 μM Incubation Duration: 48 hrs (hours) Experimental Results: Induction of cell cycle arrest and cell death. |
| Animal Protocol |
Animal/Disease Models: Female balb/c (Bagg ALBino) mouse were injected with JC mouse breast cancer cells [1]
Doses: 20 mg/kg or 50 mg/kg Route of Administration: intraperitoneal (ip) injection; one time/day, 5 days a week; for 3 weeks. Experimental Results: Same Delayed tumor growth in genetic tumor models. |
| References |
| Molecular Formula |
C₂₅H₁₉NO₃
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|---|---|
| Molecular Weight |
381.42
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| Exact Mass |
381.136
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| CAS # |
1402830-75-4
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| Appearance |
White to yellow solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
629.2±55.0 °C at 760 mmHg
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| Flash Point |
334.4±31.5 °C
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| Vapour Pressure |
0.0±1.8 mmHg at 25°C
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| Index of Refraction |
1.657
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| LogP |
4.9
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| Synonyms |
Ceranib2; Ceranib 2
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~131.09 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (6.55 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (6.55 mM) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6218 mL | 13.1089 mL | 26.2178 mL | |
| 5 mM | 0.5244 mL | 2.6218 mL | 5.2436 mL | |
| 10 mM | 0.2622 mL | 1.3109 mL | 2.6218 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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