Size | Price | Stock | Qty |
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5mg |
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10mg |
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25mg |
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50mg |
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100mg |
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250mg |
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Other Sizes |
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Purity: ≥98%
CFSE [5(6)-Carboxyfluorescein diacetate succinimidyl ester; CFDA-SE; 5(6)-CFDA N-succinmidyl ester] is a novel, a cell-permeable and amine-reactive fluorescent dye which has been widely used to track cell division by covalently coupling, via its succinimidyl group, to intracellular molecules such as lysine residues and other amine sources. Specifically, it is used to monitor distinct generations of proliferating cells by dye dilution. It is non-fluorescent in the parent form until the acetate groups are cleaved by intracellular esterases to produce the highly fluorescent fluorophore.
ln Vitro |
Composition of CFDA-SE working solution 1.1 Composition of stock solution It is recommended to dissolve 1 milligram CFDA-SE in 0.1794 mL DMSO to achieve 10 mM CFDA-SE. Note: Store the stock solution at -20℃ or -80℃ in the dark to avoid repeated freezing. 1.2 Concentration of CFDA-SE working solution. Inducing stock solution in serum-free cell culture. Note: Please adjust the concentration of CFDA-SE working solution according to the real situation. Cell staining 2.1 Suspended cells: Centrifuge at 1000 g for 3-5 minutes at 4°C and discard the supernatant. Use PBS to wash twice, for five minutes each time. Cell wall-attached cells: Discard the cell culture media, apply trypsin to separate the cells and produce a single suspension. Centrifuge at 1000 g for 3-5 minutes at 4°C and discard the supernatant. Use PBS to wash twice, for five minutes each time. 2.2 Add 1 mL CFDA-SE working solution and mix for 30 minutes. 2.3 Centrifuge at 400 g for 3-4 minutes at 4°C. 2.4 Use PBS twice, giving each wash five minutes. 2.5 Use serum-free cells or cells resuspended in PBS, and detect using fluorescence microscopy or flow cytometer.
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References |
[1]. Banks HT, et al. Quantifying CFSE Label Decay in Flow Cytometry Data. Appl Math Lett. 2013 May 1;26(5):571-577.
[2]. A Bruce Lyons, et al. Flow cytometric analysis of cell division by dilution of CFSE and related dyes. Curr Protoc Cytom. 2013;Chapter 9:Unit9.11. [3]. Cui J, et al. Prostaglandin E3 attenuates macrophage-associated inflammation and prostate tumour growth by modulating polarization. J Cell Mol Med. 2021 Jun;25(12):5586-5601. [4]. Xiuyun Jiang et al. Long-lived pancreatic ductal adenocarcinoma slice cultures enable precise study of the immune microenvironment. Oncoimmunology. 2017; 6(7): e1333210 |
Molecular Formula |
C₂₉H₁₉NO₁₁
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Molecular Weight |
557.46
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CAS # |
150347-59-4
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
CC(OC1=CC=C(C2(C(C=CC(C(ON3C(CCC3=O)=O)=O)=C4)=C4C(O2)=O)C(C=CC(OC(C)=O)=C5)=C5O6)C6=C1)=O.CC(OC7=CC=C(C8(C(C=C(C(ON9C(CCC9=O)=O)=O)C=C%10)=C%10C(O8)=O)C(C=CC(OC(C)=O)=C%11)=C%11O%12)C%12=C7)=O;
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Synonyms |
5(6-Carboxyfluorescein diacetate succinimidyl ester; CFDA-SE; 5(6-CFDA N-succinmidyl ester;
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~50 mg/mL (~89.69 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (3.73 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (3.73 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.7939 mL | 8.9693 mL | 17.9385 mL | |
5 mM | 0.3588 mL | 1.7939 mL | 3.5877 mL | |
10 mM | 0.1794 mL | 0.8969 mL | 1.7939 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.