Size | Price | Stock | Qty |
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5mg |
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10mg |
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25mg |
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50mg |
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100mg |
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250mg |
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Other Sizes |
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ln Vitro |
Chelidonine (5, 10 and 20 μM; 3–4 days) resulted in lesions and ventral curling in Dugesia japonica; at 20 μM, Djmcm2 expression was greatly reduced, but at 5 and 10 μM, no reduction was seen; also, it blocked stem cells' ability to advance through the cell cycle [2]. Melanoma cell lines are susceptible to the cytotoxic effects of chelidonine (0–3 μg/mL; 48 hours) [3]. In A-375 cells, the mitochondrial membrane potential (MMP) was 50% lower at 1 and 1.5 μg/mL and at 3 μg/mL of chelidonine (1, 2, and 3 μg/mL; 24 hours). 62% decrease [3].
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Cell Assay |
Cytotoxicity assay [3]
Cell Types: A-375, A-375-p53DD and A-375-p53sh Tested Concentrations: 0-3 μg/mL Incubation Duration: 48 hrs (hours) Experimental Results: demonstrated cytotoxic activity against melanoma cell lines, The values were 0.910±0.017 μg/ml, 0.634±0.009 μg/ml and 0.772±0.045 μg/ml in A-375, A-375-p53DD and A-375-p53sh, respectively. |
References |
[1]. Lee YK, et al. Chelidonine Induces Caspase-Dependent and Caspase-Independent Cell Death through G2/M Arrest in the T98G Human Glioblastoma Cell Line. Evid Based Complement Alternat Med. 2019 May 26;2019:6318179.
[2]. Isolani ME, et al. The in vivo effect of chelidonine on the stem cell system of planarians. Eur J Pharmacol. 2012 Jul 5;686(1-3):1-7. [3]. Hammerová J, et al. Benzo[c]phenanthridine alkaloids exhibit strong anti-proliferative activity in malignant melanoma cells regardless of their p53 status. J Dermatol Sci. 2011 Apr;62(1):22-35. |
Molecular Formula |
C20H19NO5
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Molecular Weight |
353.37
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Exact Mass |
353.13
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CAS # |
476-32-4
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
O([H])[C@@]1([H])C([H])([H])C2=C([H])C3=C(C([H])=C2[C@]2([H])[C@@]1([H])C1C([H])=C([H])C4=C(C=1C([H])([H])N2C([H])([H])[H])OC([H])([H])O4)OC([H])([H])O3.O([H])[H]
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Synonyms |
Chelidonine Khelidonin Stylophorin Stylophorin
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~100 mg/mL (~282.99 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (7.07 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (7.07 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (7.07 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.8299 mL | 14.1495 mL | 28.2990 mL | |
5 mM | 0.5660 mL | 2.8299 mL | 5.6598 mL | |
10 mM | 0.2830 mL | 1.4149 mL | 2.8299 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.