Size | Price | Stock | Qty |
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50mg |
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100mg |
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250mg |
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500mg |
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1g |
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Other Sizes |
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Purity: ≥98%
Chrysophanic acid (also called Chrysophanol), a naturally occuring anthraquinone isolated from Dianella longifolia, is a selective and potent inhibitor of EGFR/mTOR (epidermal growth factor (EGF) receptor/mammalian target of rapamycin) with potential anti-obesity and antitumor activity. In C57BL/6 mice, a high-fat diet (HFD)-induced obesity is ameliorated by chyrsophanol (CA). Additionally, in EGFR-overexpressing SNU-C5 human colon cancer cells, it demonstrated strong antiproliferative and anticancer activity. It did not affect other cell lines with low levels of EGFR expression, but it specifically inhibited the proliferation of SNU-C5 cells.
Targets |
EGFR; mTOR
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ln Vitro |
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ln Vivo |
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Cell Assay |
In 96-well microplates, the cells are seeded at 5×103 cells/mL and given 24 hours to attach. The medium is supplemented with chrysophanol (20, 50, 80, and 120 μM) at varying concentrations up to 120 μM and for varying amounts of time. A Cell Counting Kit-8 is used to measure the cytotoxicity and/or proliferation of treated cells (CCK-8). In a nutshell, formazan, an orange-colored water-soluble product, is produced by the highly water-soluble tetrazolium salt WST-8. The number of living cells is exactly proportional to the amount of formazan dye produced by cell dehydrogenases. A microplate reader is used to measure the absorbance at 450 nm to determine the cytotoxicity and proliferation of cells after adding 10 μL of CCK-8 to each well and letting it sit at 37°C for three hours. For every experimental condition, three replicated wells are used[1].
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Animal Protocol |
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References |
Molecular Formula |
C15H10O4
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Molecular Weight |
254.24
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Exact Mass |
254.06
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Elemental Analysis |
C, 70.86; H, 3.96; O, 25.17
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CAS # |
481-74-3
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Related CAS # |
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Appearance |
Solid powder
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SMILES |
CC1=CC2=C(C(=C1)O)C(=O)C3=C(C2=O)C=CC=C3O
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InChi Key |
LQGUBLBATBMXHT-UHFFFAOYSA-N
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InChi Code |
InChI=1S/C15H10O4/c1-7-5-9-13(11(17)6-7)15(19)12-8(14(9)18)3-2-4-10(12)16/h2-6,16-17H,1H3
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Chemical Name |
1,8-dihydroxy-3-methylanthracene-9,10-dione
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Synonyms |
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
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Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.9333 mL | 19.6665 mL | 39.3329 mL | |
5 mM | 0.7867 mL | 3.9333 mL | 7.8666 mL | |
10 mM | 0.3933 mL | 1.9666 mL | 3.9333 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.