Clindamycin phosphate

Alias: Cleocin T; Clinda-Derm; Clindagel; Evoclin; U 28508; U-28508; U28508

Cat No.:V18487 Purity: ≥98%

COA Product Data Instructions for use SDS

Clindamycin phosphate (EC2443986; U 21251 F;U-28508;U-21251),the phosphate salt ofClindamycin which is an antibiotic, is a potent ribosomal translocation and protein synthesis inhibitor.

Clindamycin phosphate Chemical Structure CAS No.: 24729-96-2
Product category: Bacterial

This product is for research use only, not for human use. We do not sell to patients.

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Other Forms of Clindamycin phosphate:

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Product Description
Bioactivity & Protocols
Physicochemical Properties
Solubility Data
Calculators
Biological Data

Product Description

Clindamycin phosphate (Clindamycin 2-phosphate) is a broad-spectrum bacteriostatic lincosamide antibiotic. The proagent of Clindamycin (HY-B1455), clindamycin phosphate, has no antimicrobial activity in vitro but can be quickly hydrolyzed by phosphatase ester in vivo to become the active parent agent, Clindamycin. One can use clindamycin phosphate to study bacterial vaginosis and acne.

Biological Activity I Assay Protocols (From Reference)

Targets	Antibiotic, Bacterial
References	[1]. Eur J Clin Pharmacol. 2017 Feb;73(2):251-253. [2]. J Dermatol. 2018 Aug;45(8):951-962. [3]. Gynecol. 2020, 47(4), 516–518. [4]. Ann Clin Microbiol Antimicrob. 2018 Oct 20;17(1):38.
Additional Infomation	Clindamycin Phosphate is the phosphate salt form of clindamycin, a semi-synthetic, chlorinated broad spectrum antibiotic produced by chemical modification of lincomycin. Clindamycin phosphate is used in topical preparations. See also: Clindamycin (has active moiety); Benzoyl Peroxide; Clindamycin Phosphate (component of); Clindamycin phosphate; tretinoin (component of) ... View More ...

These protocols are for reference only. InvivoChem does not independently validate these methods.

Physicochemical Properties

Molecular Formula	C18H34CLN2O8PS
Molecular Weight	504.95
Exact Mass	504.146
Elemental Analysis	C, 42.81; H, 6.79; Cl, 7.02; N, 5.55; O, 25.35; P, 6.13; S, 6.35
CAS #	24729-96-2
Related CAS #	Clindamycin hydrochloride;21462-39-5;Clindamycin palmitate hydrochloride;25507-04-4;Clindamycin;18323-44-9;Clindamycin phosphate hydrochloride;64023-51-4
PubChem CID	443385
Appearance	White to off-white solid powder.
Density	1.4±0.1 g/cm3
Boiling Point	159°C
Melting Point	114ºC
Index of Refraction	1.577
LogP	0.93
Hydrogen Bond Donor Count	5
Hydrogen Bond Acceptor Count	10
Rotatable Bond Count	9
Heavy Atom Count	31
Complexity	658
Defined Atom Stereocenter Count	9
SMILES	Cl[C@@]([H])(C([H])([H])[H])[C@]([H])([C@]1([H])[C@@]([H])([C@@]([H])([C@]([H])([C@]([H])(O1)SC([H])([H])[H])OP(=O)(O[H])O[H])O[H])O[H])N([H])C([C@]1([H])C([H])([H])[C@@]([H])(C([H])([H])C([H])([H])C([H])([H])[H])C([H])([H])N1C([H])([H])[H])=O
InChi Key	UFUVLHLTWXBHGZ-MGZQPHGTSA-N
InChi Code	InChI=1S/C18H34ClN2O8PS/c1-5-6-10-7-11(21(3)8-10)17(24)20-12(9(2)19)15-13(22)14(23)16(18(28-15)31-4)29-30(25,26)27/h9-16,18,22-23H,5-8H2,1-4H3,(H,20,24)(H2,25,26,27)/t9-,10+,11-,12+,13+,14-,15+,16+,18+/m0/s1
Chemical Name	(2R,3R,4S,5R,6R)-6-((1S,2S)-2-chloro-1-((2S,4R)-1-methyl-4-propylpyrrolidine-2-carboxamido)propyl)-4,5-dihydroxy-2-(methylthio)tetrahydro-2H-pyran-3-yl dihydrogen phosphate
Synonyms	Cleocin T; Clinda-Derm; Clindagel; Evoclin; U 28508; U-28508; U28508
HS Tariff Code	2934.99.9001
Storage	Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Solubility Data

Solubility (In Vitro)	H2O : 100 mg/mL (198.04 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 50 mg/mL (99.02 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication. (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.9804 mL	9.9020 mL	19.8039 mL
	5 mM	0.3961 mL	1.9804 mL	3.9608 mL
	10 mM	0.1980 mL	0.9902 mL	1.9804 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

Calculator

Mass

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Molarity Calculator allows you to calculate the mass, volume, and/or concentration required for a solution, as detailed below:

Calculate the Mass of a compound required to prepare a solution of known volume and concentration
Calculate the Volume of solution required to dissolve a compound of known mass to a desired concentration
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See Example

An example of molarity calculation using the molarity calculator is shown below:
What is the mass of compound required to make a 10 mM stock solution in 5 ml of DMSO given that the molecular weight of the compound is 350.26 g/mol?

Enter 350.26 in the Molecular Weight (MW) box
Enter 10 in the Concentration box and choose the correct unit (mM)
Enter 5 in the Volume box and choose the correct unit (mL)
Click the “Calculate” button
The answer of 17.513 mg appears in the Mass box. In a similar way, you may calculate the volume and concentration.

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Dilution Calculator allows you to calculate how to dilute a stock solution of known concentrations. For example, you may Enter C1, C2 & V2 to calculate V1, as detailed below:

What volume of a given 10 mM stock solution is required to make 25 ml of a 25 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=25 μM, V2=25 ml and V1 is the unknown:

Enter 10 into the Concentration (Start) box and choose the correct unit (mM)
Enter 25 into the Concentration (End) box and select the correct unit (mM)
Enter 25 into the Volume (End) box and choose the correct unit (mL)
Click the “Calculate” button
The answer of 62.5 μL (0.1 ml) appears in the Volume (Start) box

Molecular formula

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Molecular Weight Calculator allows you to calculate the molar mass and elemental composition of a compound, as detailed below:

Note: Chemical formula is case sensitive: C12H18N3O4 c12h18n3o4
Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter the chemical/molecular formula and click the “Calculate’ button.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (or molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

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Reconstitution Calculator allows you to calculate the volume of solvent required to reconstitute your vial.

Enter the mass of the reagent and the desired reconstitution concentration as well as the correct units
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The answer appears in the Volume (to add to vial) box

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal to make allowance for loss during the experiment)

Dosage mg/kg

Average weight of animals g

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Step 2: Enter in vivo formulation (This is only a calculator, not the exact formulation for a specific product. Please contact us first if there is no in vivo formulation in the solubility section.)

% DMSO

% Tween 80

% ddH₂O

Calculation results

Working concentration： mg/mL；

Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O，mix and clarify.

Note： (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.

Biological Data

The adjusted mean percentage reduction from baseline over 12 weeks in (a) total lesion (TL), (b) inflammatory lesion (IL) and (c) non‐IL counts. [2]. J Dermatol. 2018 Aug;45(8):951-962.

Proportion of patients with (a) at least a two‐grade improvement in Investigator's Static Global Assessment (ISGA) score or (b) with an Investigator's Static Global Assessment (ISGA) score of 0 or 1 at each study visit. [2]. J Dermatol. 2018 Aug;45(8):951-962.

Skindex‐16 assessment scores (a) total, (b) symptom, (c) emotion and (d) functioning domains.[2]. J Dermatol. 2018 Aug;45(8):951-962.