Size | Price | Stock | Qty |
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100mg |
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500mg |
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1g |
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5g |
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10g |
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Other Sizes |
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Purity: ≥98%
Clomipramine HCl (G-34586; G34586; Trade names: Anafranil, Clomicalm), the hydrochloride salt of clomipramine which is a tricyclic antidepressant (TCA), is a potent blocker of serotonin transporter (SERT), norepinephrine transporter (NET) dopamine transporter (DAT) with Ki of 0.14, 54 and 3 nM, respectively. Clomipraminehas been used to treat chronic pain, major depressive disorder, panic disorder, and obsessive compulsive disorder.
Targets |
5-HT
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ln Vitro |
Clomipramine hydrochloride inhibits both norreplenishing and 5-HT replenishment, although clomipramine hydrochloride inhibits 5-HT replenishment more than it inhibits norreplenishing replenishment [1]. It is concentration-dependent, but not for AChE in the brain striatum [2]. Clomipramine hydrochloride interferes with autophagy attenuation and severely impairs the viability of tumor cells leading to cytotoxicity [3]. Clomipramine hydrochloride reduces autophagy in primary neuronal cultures. Clomipramine hydrochloride (1-5 μM) negatively regulates neuronal autophagy staining in cultured primary cells [3]. Western Blot Analysis[3] Cell Line: Primary Cortical Neurons Concentration: 1 and 5 µM Incubation Time: 12, 24 and 48 hours Results: Enhanced LC3-I to LC3-II in a concentration-dependent manner at all analyzed time points transformation.
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ln Vivo |
Clomipramine Hydrochloride (5 -20 mg/kg; ip) The hydrochloride salt causes significant hypertension in the nozzle. Clomipramine Hydrochloride Promotes Electrode Release by Burning 5-HT2B and/or 5-HT2C Bus Adapters Hypertension In rats, clomipramine hydrochloride reduces immobility in the forced swim test, which is inhibitory to drug resistance. behavioral model. Clomipramine hydrochloride also inhibits the marble burying behavior of rats, an animal model of obsessive-compulsive disorder [1]. Clomipramine (20 mg/animal model: C57BL/6 J mice (6 weeks old, 22 to 25 g) [3] Dose: 20 mg/kg Administration: intraperitoneal injection for 21 days Results: vs. vehicle Both LC3-II and p62 were significantly increased in the livers of clomipramine-treated mice compared with clomipramine-treated mice.
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Cell Assay |
Cell Line: Primary cortical neurons
Concentration: 1 and 5 µM Incubation Time: 12, 24 and 48 hours Result: Enhanced the LC3-I conversion to LC3-II in a concentration-dependent manner at all analyzed time points. |
Animal Protocol |
C57BL/6 J mice (6 weeks of age and 22 to 25 g)
20 mg/kg Treated intraperitoneally for 21 days |
References |
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Molecular Formula |
C19H24CL2N2
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Molecular Weight |
351.31
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Exact Mass |
350.13
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Elemental Analysis |
C, 64.96; H, 6.89; Cl, 20.18; N, 7.97
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CAS # |
17321-77-6
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Related CAS # |
Clomipramine-d3 hydrochloride; 1398065-86-5; Clomipramine; 303-49-1; Clomipramine-d3; 136765-29-2; Clomipramine-d6 hydrochloride; 1189882-28-7; Clomipramine-13C,d3 hydrochloride
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Appearance |
White to off-white crystalline powder
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SMILES |
CN(C)CCCN1C2=CC=CC=C2CCC3=C1C=C(C=C3)Cl.Cl
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InChi Key |
WIMWMKZEIBHDTH-UHFFFAOYSA-N
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InChi Code |
InChI=1S/C19H23ClN2.ClH/c1-21(2)12-5-13-22-18-7-4-3-6-15(18)8-9-16-10-11-17(20)14-19(16)22;/h3-4,6-7,10-11,14H,5,8-9,12-13H2,1-2H3;1H
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Chemical Name |
3-(2-chloro-5,6-dihydrobenzo[b][1]benzazepin-11-yl)-N,N-dimethylpropan-1-amine;hydrochloride
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Synonyms |
Clomimipramine; G 34586; 3-Chloroimipramine; G-34586; G34586; Trade names: Anafranil, Clomicalm
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 3 mg/mL (8.54 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 30.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 3 mg/mL (8.54 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 30.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 3 mg/mL (8.54 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 100 mg/mL (284.65 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.8465 mL | 14.2324 mL | 28.4649 mL | |
5 mM | 0.5693 mL | 2.8465 mL | 5.6930 mL | |
10 mM | 0.2846 mL | 1.4232 mL | 2.8465 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
NCT Number | Recruitment | interventions | Conditions | Sponsor/Collaborators | Start Date | Phases |
NCT01575158 | Completed | Drug: Citalopram Drug: Clomipramine Drug: placebo |
Depression | University of Aarhus | October 1997 | Not Applicable |
NCT02028598 | Completed | Drug: Treatment 1 Drug: Treatment 2 Drug: Treatment 3 |
Healthy | CTC Bio, Inc. | January 2014 | Phase 1 |
NCT00254735 | Completed | Drug: quetiapine fumarate Drug: SSRI/Clomipramine |
Obsessive Compulsive Disorder | AstraZeneca | April 2002 | Phase 3 |
NCT01439984 | Completed | Drug: Clomipramine | Premature Ejaculation | Symyoo | September 2011 | Phase 3 |
NCT00564564 | Completed | Drug: Quetiapine Drug: Clomipramine |
Obsessive Compulsive Disorder | University of Sao Paulo | January 2006 | Phase 4 |
Clomipramine and fluoxetine treatments reduce autophagy flux in cortical neurons. Sci Rep . 2019 Mar 19;9(1):4881. td> |
Autophagic flux is decreased by clomipramine treatment in mouse tissues. Sci Rep . 2019 Mar 19;9(1):4881. td> |
Clomipramine and fluoxetine modulate C. elegans autophagy in a different manner. Sci Rep . 2019 Mar 19;9(1):4881. td> |
The graphs show rat brain striatum acetylcholinesterase experiments in the presence of 0.5 mM different antidepressants (Clom; clomipramine, Sert; sertraline, Paro; paroxetine and Imip; imipramine). J Enzyme Inhib Med Chem . 2008 Dec;23(6):912-7. td> |