| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
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| ln Vitro |
Col001 (AK-778) is split into Col003 (4.4–40 μM). Col003 on Hsp47 KO/Col003 (0.01-100 μM) exhibits effects on collagen responses and Hsp47 that are dose-dependent. Hsp47 has an IC50 value of 1.8 μM[1]. There is an inhibitory effect on the growth and accumulation of collagen at 100 μM (20–60 minutes). In wild-type MEFs, Col003 (100 μM) modifies puppy expression without totally disappearing [1]. In wild-type MEFs, Col003 (100 μM) modifies puppy expression without totally disappearing [1]. (50°C for 15 minutes), but at 37°C, it cannot be broken down by islet digestion [1].
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|---|---|
| Cell Assay |
Western Blot Analysis [1]
Cell Types: Wild-type MEF Tested Concentrations: 100μM Incubation Duration: 50°C or then 37°C for 15 minutes Experimental Results: Correctly folded triple helix structure and the effect of α,α'-bipyridyl on collagen secretion. |
| References |
| Molecular Formula |
C14H11NO4
|
|---|---|
| Molecular Weight |
257.241443872452
|
| Exact Mass |
257.068
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| CAS # |
328565-16-8
|
| PubChem CID |
871077
|
| Appearance |
Light yellow to yellow solid powder
|
| LogP |
3.3
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
19
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| Complexity |
322
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| Defined Atom Stereocenter Count |
0
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| SMILES |
OC1C(C=O)=CC(=CC=1[N+](=O)[O-])CC1C=CC=CC=1
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| InChi Key |
RKJXVCLOGNLZOI-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C14H11NO4/c16-9-12-7-11(6-10-4-2-1-3-5-10)8-13(14(12)17)15(18)19/h1-5,7-9,17H,6H2
|
| Chemical Name |
5-benzyl-2-hydroxy-3-nitrobenzaldehyde
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~194.37 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (9.72 mM) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.8874 mL | 19.4371 mL | 38.8742 mL | |
| 5 mM | 0.7775 mL | 3.8874 mL | 7.7748 mL | |
| 10 mM | 0.3887 mL | 1.9437 mL | 3.8874 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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