Size | Price | Stock | Qty |
---|---|---|---|
50mg |
|
||
100mg |
|
||
Other Sizes |
|
ln Vitro |
Compound 48/80 trihydrochloride, also known as C48/80 trihydrochloride, exhibits a comparable level of efficacy in inhibiting phosphatidylphospholipidase C activity in the cytosol; its IC50 values are 2.1 μg/mL for the supernatant and 5.0 μg/mL for the granular compound. Additionally, it has been observed that C48/80 trihydrochloride inhibits human focal aggregation induced by ADP and PAF ether [1].
|
---|---|
ln Vivo |
Serotonin, histamine, and corticosterone levels are increased by compound 48/80 trihydrochloride (0.75 mg/kg; intraperitoneal; killed after 0.5, 3, or 6 h); however, the amount of these increases decreases after 0.5 h [3]. Compound 48/80 trihydrochloride induces redox through mast cell degranulation [3].
|
Animal Protocol |
Animal/Disease Models: 7weeks old Wistar male rats (fasted for 24 hrs (hrs (hours))) [3]
Doses: 0.75 mg/kg]. Route of Administration: intraperitoneal (ip) injection; sacrificed after 0.5, 3 or 6 hrs (hrs (hours)). Experimental Results: 0.5 hrs (hrs (hours)) after treatment, serum histamine and serotonin concentrations were Dramatically higher than untreated control rats. Serum histamine and serotonin concentrations increased in rats and subsequently diminished in a time-dependent manner. |
References |
[1]. Bronner C, et al. Compound 48/80 is a potent inhibitor of phospholipase C and a dual modulator of phospholipase A2 from human platelet. Biochim Biophys Acta. 1987 Aug 15;920(3):301-5.
[2]. Schemann M, et al. The mast cell degranulator compound 48/80 directly activates neurons. PLoS One. 2012;7(12):e52104. [3]. Kaida S, et al. Compound 48/80 causes oxidative stress in the adrenal gland of rats through mast cell degranulation. Free Radic Res. 2010 Feb;44(2):171-80. |
Molecular Formula |
C32H48CL3N3O3
|
---|---|
Molecular Weight |
629.100826263428
|
CAS # |
848035-21-2
|
Related CAS # |
Compound 48/80;94724-12-6
|
Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
|
SMILES |
C1(CCNC)C=C(CC2C(OC)=C(CC3C=C(CCNC)C=CC=3OC)C=C(CCNC)C=2)C(OC)=CC=1.Cl.Cl.Cl
|
HS Tariff Code |
2934.99.9001
|
Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
Solubility (In Vitro) |
H2O : ~25 mg/mL (~39.74 mM)
DMSO : ~10 mg/mL (~15.90 mM) |
---|---|
Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (3.97 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (3.97 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.5896 mL | 7.9479 mL | 15.8957 mL | |
5 mM | 0.3179 mL | 1.5896 mL | 3.1791 mL | |
10 mM | 0.1590 mL | 0.7948 mL | 1.5896 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.