Size | Price | Stock | Qty |
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5mg |
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10mg |
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25mg |
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50mg |
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100mg |
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250mg |
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500mg |
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Other Sizes |
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Purity: ≥98%
CW069 (CW-069; CW 069) is a potent, selective and allosteric inhibitor of microtubule motor protein HSET with potential antitumor activity. It inhibits HSET with an IC50 of 75 μM.
ln Vitro |
CW-069 is an HSET allosteric inhibitor, with an IC50 of 75 μM. Selectivity over KSP that is statistically significant is demonstrated by CW-069. CW-069 exhibits a robust suppressive effect on N1E-115 cells and a less potent inhibitory effect on NHDF cells, with IC50 values of 86 ± 10 μM and 181 ± 7 μM, respectively. When applied in N1E-115 cells with supernumerary centrosomes, CW-069 (100 or 200 μM) increases multipolar spindles, but it has no effect on the shape of bipolar spindles in normal human dermal fibroblast cells. While CW-069 (200 μM) inhibits monastrol's suppression of KSP and causes multipolar anaphase and cell death in N1E-115 cells transfected with HSET siRNA, it does not cause mitotic arrest in HeLa cells[1].
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ln Vivo |
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Animal Protocol |
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References |
Molecular Formula |
C23H21IN2O3
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Molecular Weight |
500.33
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Exact Mass |
500.059
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CAS # |
1594094-64-0
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Related CAS # |
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PubChem CID |
73427517
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Appearance |
White to off-white solid powder
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Density |
1.6±0.1 g/cm3
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Boiling Point |
666.0±55.0 °C at 760 mmHg
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Flash Point |
356.6±31.5 °C
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Vapour Pressure |
0.0±2.1 mmHg at 25°C
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Index of Refraction |
1.687
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LogP |
6.74
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Hydrogen Bond Donor Count |
3
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Hydrogen Bond Acceptor Count |
4
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Rotatable Bond Count |
8
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Heavy Atom Count |
29
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Complexity |
532
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Defined Atom Stereocenter Count |
1
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SMILES |
C1=CC=C(C=C1)C[C@@H](C(=O)NC2=C(C=C(C=C2)I)C(=O)O)NCC3=CC=CC=C3
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InChi Key |
IRDIXDXDSUBHIU-NRFANRHFSA-N
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InChi Code |
InChI=1S/C23H21IN2O3/c24-18-11-12-20(19(14-18)23(28)29)26-22(27)21(13-16-7-3-1-4-8-16)25-15-17-9-5-2-6-10-17/h1-12,14,21,25H,13,15H2,(H,26,27)(H,28,29)/t21-/m0/s1
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Chemical Name |
5-Iodo-2-[[(2S)-1-oxo-3-phenyl-2-[(phenylmethyl)amino]propyl]amino]-benzoic acid
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Synonyms |
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (4.16 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (4.16 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (4.16 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.9987 mL | 9.9934 mL | 19.9868 mL | |
5 mM | 0.3997 mL | 1.9987 mL | 3.9974 mL | |
10 mM | 0.1999 mL | 0.9993 mL | 1.9987 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.