Size | Price | Stock | Qty |
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5mg |
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10mg |
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25mg |
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50mg |
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Other Sizes |
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ln Vitro |
Guide (the following is our proposed plan, this plan merely gives a guide and can be modified according to your individual needs). Binding of SELP analogues to Cy5-SE [1]. 1. Take 1 mL of 100 mM sodium bicarbonate buffer. 2. Quench reaction: mix 50 μL of 100 μg/mL SELP solution made of 1 M (pH 8.3) and 10 μL of 1.2 mg/mL Cy5 monomer composed of 10% DMSO. NHS ester was combined and kept on ice for 2 hours. 3. Load the reaction mixture onto a 1.5 mL Sephadex G-25 column, and centrifuge at 1050 g for 3 minutes to elute Cy5-labeled SELP. Note: 1. CY dye will not significantly interfere with peptides 2. Low amounts of sodium azide (≤3 mM or 0.02%) or thimerosal (≤0.02 mM or 0.01%). 3. Avoid utilizing buffers containing primary amines (such as Tris, glycine) or ammonium ions. They will compete with the protein to be tagged and impair labeling efficiency. 4. For dyes without activator, you need to use condensation solution (500 ug/mL) (25952-53-8) activator candle for future marking tests. 5. This product is solely for scientific research by professionals and may not be used for clinical diagnosis or treatment. 6. For your safety and health, please don a lab coat and wear a disposable overview for operation.
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References |
Molecular Formula |
C₃₇H₄₃N₃O₁₀S₂
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Molecular Weight |
753.88
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Exact Mass |
753.239
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CAS # |
146368-14-1
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Related CAS # |
CY5-SE triethylamine salt;1497420-70-8
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PubChem CID |
126969923
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Appearance |
Dark purple to black solid powder
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LogP |
7.318
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Hydrogen Bond Donor Count |
1
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Hydrogen Bond Acceptor Count |
11
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Rotatable Bond Count |
13
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Heavy Atom Count |
52
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Complexity |
1720
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Defined Atom Stereocenter Count |
0
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InChi Key |
WXWLHDCCGVWTDZ-UHFFFAOYSA-N
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InChi Code |
InChI=1S/C37H43N3O10S2/c1-6-38-29-18-16-25(51(44,45)46)23-27(29)36(2,3)31(38)13-9-7-10-14-32-37(4,5)28-24-26(52(47,48)49)17-19-30(28)39(32)22-12-8-11-15-35(43)50-40-33(41)20-21-34(40)42/h7,9-10,13-14,16-19,23-24H,6,8,11-12,15,20-22H2,1-5H3,(H-,44,45,46,47,48,49)
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Chemical Name |
2-[5-[1-[6-(2,5-dioxopyrrolidin-1-yl)oxy-6-oxohexyl]-3,3-dimethyl-5-sulfoindol-1-ium-2-yl]penta-2,4-dienylidene]-1-ethyl-3,3-dimethylindole-5-sulfonate
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Synonyms |
Cy5 NHS Ester Sulfo-Cyanine5 Succinimidyl Ester
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~25 mg/mL (~33.16 mM)
H2O : ≥ 5.88 mg/mL (~7.80 mM) |
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (3.32 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (3.32 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: 25 mg/mL (33.16 mM) in Saline (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication (<60°C). |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.3265 mL | 6.6324 mL | 13.2647 mL | |
5 mM | 0.2653 mL | 1.3265 mL | 2.6529 mL | |
10 mM | 0.1326 mL | 0.6632 mL | 1.3265 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.