| Size | Price | Stock | Qty |
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| 5mg |
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| 25mg |
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Purity: ≥98%
Decoyinine (also known as Angustmycin A) is a potent and selective inhibitor of GMP synthetase (GMPS, guanosine monophosphate synthase). Decoyinine allows sporulation in Bacillus subtilis to initiate and proceed under otherwise catabolite-repressing conditions. Decoyinine did not overcome catabolite repression of alpha-amylase synthesis in a wild-type strain of B. subtilis but did cause premature and enhanced synthesis in a mutant strain specifically blocked in catabolite repression of alpha-amylase synthesis. Decoyinine had no effect on alpha-amylase enzymatic activity. Thus, it appears that the catabolite control mechanisms governing alpha-amylase synthesis and sporulation in B. subtilis differ in their responses to decoyinine and hence must consist at least partially of separate components.
| ln Vitro |
Decoyinine, an adenosine analogue, inhibits GMP synthase, resulting in a drop in intracellular GTP levels. Decoyinine only stimulates the synthesis of oa amylase in strains whose gra-10 mutations have rendered downstream regulatory components of α-amylase control unworkable. Decoyinine's apparent enhancement of a-amylase activity in WLN-11 is not a result of variations in the enzyme's activity level. Purine nucleotides, particularly GMP, have been shown to have strong inhibitory effects on B's activity. amylase frontis F-2. At 1.07 mM, the concentration utilized in the research, Decoyinine did not affect B's activity. α-amylase from subtilis WLN-11 in vitro. GMP slightly inhibits α-amylase activity at the same concentration [2].
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| ln Vivo |
Decoyinine also inhibits the growth of xenografts made from cells with NRASQ61R (SK-Mel-103) or BRAFV600E (SK-Mel-28) mutations, suggesting that its effects, like those of GMPS activity, do not appear to be subtype potential [1].
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| References |
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| Additional Infomation |
Decoyinine is a member of 6-aminopurines.
Decoyinine has been reported in Streptomyces angustmyceticus with data available. |
| Molecular Formula |
C11H13N5O4
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| Molecular Weight |
279.25202
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| Exact Mass |
279.096
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| CAS # |
2004-04-8
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| Related CAS # |
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| PubChem CID |
121578
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| Appearance |
Typically exists as solid at room temperature
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| Density |
1.9±0.1 g/cm3
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| Boiling Point |
580.3±60.0 °C at 760 mmHg
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| Flash Point |
304.7±32.9 °C
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| Vapour Pressure |
0.0±1.7 mmHg at 25°C
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| Index of Refraction |
1.837
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| LogP |
-1.25
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
20
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| Complexity |
410
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| Defined Atom Stereocenter Count |
3
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| SMILES |
C=C(O1)[C@@H](O)[C@@H](O)[C@]1(CO)N2C=NC3=C(N)N=CN=C32
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| InChi Key |
UZSSGAOAYPICBZ-SOCHQFKDSA-N
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| InChi Code |
InChI=1S/C11H13N5O4/c1-5-7(18)8(19)11(2-17,20-5)16-4-15-6-9(12)13-3-14-10(6)16/h3-4,7-8,17-19H,1-2H2,(H2,12,13,14)/t7-,8-,11-/m1/s1
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| Chemical Name |
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| Synonyms |
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.5810 mL | 17.9051 mL | 35.8102 mL | |
| 5 mM | 0.7162 mL | 3.5810 mL | 7.1620 mL | |
| 10 mM | 0.3581 mL | 1.7905 mL | 3.5810 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
GMPS is upregulated during melanoma progression.Cell Death Differ.2015 Nov;22(11):1858-64. |
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Angustmycin A treatment affects melanoma invasionin vitroand xenograft growthin vivo.Cell Death Differ.2015 Nov;22(11):1858-64. |
GMPS contributes to the invasive capability of melanoma cells.Cell Death Differ.2015 Nov;22(11):1858-64. |
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