Size | Price | Stock | Qty |
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10mg |
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25mg |
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50mg |
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Other Sizes |
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Demecolcine (Colcemid), a colchicine analog, is a potent microtubule polymerization inhibitor / mitotic inhibitor /depolymerizing anti-tubulin agent with anticancer activity. It is an alkaloid found naturally in Colchicum autumnale L. that has been isolated from the plant.
ln Vitro |
In mice, germ cells, and frog spermatocytes, colcemid (Demecolcine) (0.1-0.25 μg/ml, 1 hour) lowers the frequency of hypoploidy of phase II complement [3].
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ln Vivo |
The incidence of poor ploidy in stage II division is increased in mice treated with intraperitoneal injection of Demecolcine (Colcemid; 0.3 mg/kg) [3].
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Animal Protocol |
Animal/Disease Models: Mice [3]
Doses: 0.3 mg/kg Route of Administration: intraperitoneal (ip) injection Experimental Results: The frequency of hypoploidy and hyperploidy in metaphase II cells increased 7.8-8 times compared to the control. |
References |
[1]. T Tsuchida, et al. Colcemid-induced apoptosis of cultured human glioma: electron microscopic and confocal laser microscopic observation of cells sorted in different phases of cell cycle. Cytometry. 1998 Apr 1;31(4):295-9.
[2]. Ashley M Rozario, et al. Ultra-Low Colcemid Doses Induce Microtubule Dysfunction as Revealed by Super-Resolution Microscopy. Bioexiv. [3]. Nett RS, et al. Discovery and engineering of colchicine alkaloid biosynthesis. Nature. 2020 Aug;584(7819):148-153. doi: 10.1038/s41586-020-2546-8. Epub 2020 Jul 22. Erratum in: Nature. 2020 Jul 30. [4]. Muzaffar A, et al. Antitubulin effects of derivatives of 3-demethylthiocolchicine, methylthio ethers of natural colchicinoids, and thioketones derived from thiocolchicine. Comparison with colchicinoids. J Med Chem. 1990 Feb;33(2):567-71. [5]. Risley MS, et al. An improved method for cytogenetic analysis of meiotic aneuploidy in rodent and frog spermatocytes. Mutat Res. 1990 Dec;234(6):361-8. |
Molecular Formula |
C21H25NO5
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Molecular Weight |
371.43
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Exact Mass |
371.1733
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Elemental Analysis |
C, 67.91; H, 6.78; N, 3.77; O, 21.54
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CAS # |
477-30-5
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Related CAS # |
477-30-5
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Appearance |
Solid powder
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SMILES |
CN[C@H]1CCC2=CC(=C(C(=C2C3=CC=C(C(=O)C=C13)OC)OC)OC)OC
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InChi Key |
NNJPGOLRFBJNIW-HNNXBMFYSA-N
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InChi Code |
InChI=1S/C21H25NO5/c1-22-15-8-6-12-10-18(25-3)20(26-4)21(27-5)19(12)13-7-9-17(24-2)16(23)11-14(13)15/h7,9-11,15,22H,6,8H2,1-5H3/t15-/m0/s1
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Chemical Name |
(7S)-1,2,3,10-tetramethoxy-7-(methylamino)-6,7-dihydro-5H-benzo[a]heptalen-9-one
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Synonyms |
C 12669; C-12669; Colcemid; Demecolcine; C12669; (-)-Demecolcine; Colchamine; Demecolcin; Colchamin
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO: ~75 mg/mL (~201.9 mM)
Ethanol: ~75 mg/mL (~201.9 mM) Water: ~75 mg/mL (~201.9 mM) |
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.73 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (6.73 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (6.73 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.6923 mL | 13.4615 mL | 26.9230 mL | |
5 mM | 0.5385 mL | 2.6923 mL | 5.3846 mL | |
10 mM | 0.2692 mL | 1.3461 mL | 2.6923 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.