| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| Other Sizes |
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| ln Vitro |
At micromolar concentrations, the water-soluble, heat-stable, non-hemolytic peptide dermaseptin exhibits strong antimicrobial activity against pathogenic fungi. Dermaseptin's 80% α-helical conformation is indicated by its circular dichroism spectra in hydrophobic media. Secondary structure predictions show that dermaseptin can be configured as an amphipathic α-helix that spans residues 1–27. This perturbation-regulated water structure of the membrane regulates flux. 1]. At micromolar concentrations, dermaseptin has a lytic effect on filamentous fungi, bacteria, yeast, and protozoa. Dermaseptin's superior antibacterial potency can be attributed to a molecular element: the NH2-terminal α-helical amphipathic fragment, which spans residues 1-18 of the molecule [1]. Dermaseptin (5-100 μg/ml; 48 hours) completely prevents the growth of the majority of tested microorganisms, including filamentous fungi, yeasts, bacteria, and parasites, at micromolar concentrations [2]. Dermaseptin treatment for one hour did not penetrate guinea pig lymphocytes up to the highest concentration assayed (200 μg/ml), and after 48 hours of incubation, it did not inhibit the proliferation of human KJ3 cells. ..Rabbit red blood cells were hemolyzed at 350 μg/ml, which resulted in a 50% hemolysis after an hour of treatment with a dose exceeding 200 μg/ml [2]. Aeromonas equi, Pseudomonas aeruginosa, Escherichia coli, Enterococcus faecalis, and L. are among the bacteria that Dermaseptin has antibacterial action against. Microsporum canis (IP1194) and mezicana (NF alpha strain) have MIC values of 50 μg/ml, 100 μg/ml, 25 μg/ml, 15 μg/ml, and 50 μg/ml, respectively [2].
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| References |
[1]. Mor A, et al. Isolation, amino acid sequence, and synthesis of dermaseptin, a novel antimicrobial peptide of amphibian skin. Biochemistry. 1991 Sep 10;30(36):8824-30.
[2]. Mor A, et al. The NH2-terminal alpha-helical domain 1-18 of dermaseptin is responsible for antimicrobial activity. J Biol Chem. 1994 Jan 21;269(3):1934-9. |
| Molecular Formula |
C152H257N43O44S2
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|---|---|
| Molecular Weight |
3455.09
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| CAS # |
136212-91-4
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| Related CAS # |
Dermaseptin TFA;646451-06-1
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| Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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| SMILES |
CC[C@@H]([C@@H](C(N[C@H](C(N[C@H](C(NCC(N[C@H](C(N[C@H](C(=O)O)CCC(=O)N)=O)[C@H](O)C)=O)=O)CCC(=O)N)=O)CO)=O)NC([C@H]([C@H](O)C)NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC(CNC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC(CNC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@H]([C@H](O)C)NC(CNC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@H]([C@H](O)C)NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](N)C)=O)CC(C)C)=O)CC1C2=C(C=CC=C2)NC=1)=O)CCCCN)=O)=O)CCSC)=O)CC(C)C)=O)CCCCN)=O)CCCCN)=O)CC(C)C)=O)=O)=O)CCSC)=O)C)=O)CC(C)C)=O)CC1N=CNC=1)=O)C)=O)=O)CCCCN)=O)C)=O)C)=O)CC(C)C)=O)=O)C)=O)C)=O)C)=O)CC(=O)O)=O)=O)C
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~25 mg/mL (~7.24 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: 50 mg/mL (14.47 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.2894 mL | 1.4471 mL | 2.8943 mL | |
| 5 mM | 0.0579 mL | 0.2894 mL | 0.5789 mL | |
| 10 mM | 0.0289 mL | 0.1447 mL | 0.2894 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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