| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
|
||
| 10mg |
|
||
| 50mg |
|
||
| 100mg |
|
||
| Other Sizes |
|
Purity: ≥98%
DFHBI-1T is a mimic of green fluorescent protein (GFP) fluorophore used for imaging RNA in living cells. Fluorescence activated by binding to Spinach2 or Broccoli aptamers. Exhibits peak excitation maxima of 482 nm and peak fluorescence emission of 505 nm when bound to Spinach2, enabling imaging with GFP filter cubes. Exhibits higher specific fluorescence and lower background fluorescence when bound to Spinach2 compared with Spinach2-DFHBI.
| ln Vitro |
In COS7 cells expressing (CGG) 60-Spinach2, DFHBI-1T (20 μM; 10 min) increases fluorescein in comparison to DFHBI (20 μM)[1]. Broccoli-DFHBI-1T has an ex/em of 472 nm/507 nm, and spinach-2-DFHBI-1T has an ex/em of 482 nm/505 nm[2].
|
|---|---|
| References |
| Molecular Formula |
C13H9F5N2O2
|
|---|---|
| Molecular Weight |
320.214780569077
|
| Exact Mass |
320.058
|
| CAS # |
1539318-36-9
|
| Related CAS # |
DFHBI-2T;1539318-40-5
|
| PubChem CID |
101889712
|
| Appearance |
Light yellow to yellow solid powder
|
| Density |
1.49±0.1 g/cm3(Predicted)
|
| Boiling Point |
327.9±52.0 °C(Predicted)
|
| Flash Point |
152.1±30.7 °C
|
| Vapour Pressure |
0.0±0.7 mmHg at 25°C
|
| Index of Refraction |
1.527
|
| LogP |
2.96
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
8
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
22
|
| Complexity |
505
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
CC1=N/C(=C\C2=CC(=C(C(=C2)F)O)F)/C(=O)N1CC(F)(F)F
|
| InChi Key |
AWYCLBWNRONMQC-WMZJFQQLSA-N
|
| InChi Code |
InChI=1S/C13H9F5N2O2/c1-6-19-10(12(22)20(6)5-13(16,17)18)4-7-2-8(14)11(21)9(15)3-7/h2-4,21H,5H2,1H3/b10-4-
|
| Chemical Name |
(5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-3-(2,2,2-trifluoroethyl)-4H-imidazol-4-one
|
| Synonyms |
DFHBI-1T DFHBI1T DFHBI 1T DF-HBI-1T DF HBI 1T
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~156.15 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 5.5 mg/mL (17.18 mM) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 55.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.1230 mL | 15.6148 mL | 31.2295 mL | |
| 5 mM | 0.6246 mL | 3.1230 mL | 6.2459 mL | |
| 10 mM | 0.3123 mL | 1.5615 mL | 3.1230 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
COA