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1g |
ln Vitro |
(+)-DHMEQ ((2R,3R,4R)-DHMEQ) stimulates Nrf2, a transcription factor that various antioxidant enzymes are induced to express. In a promoter reporter assay, (+)-DHMEQ activates Nrf2. Furthermore, DHMEQ reverses the effects of reactive oxygen species (ROS) on a neuronal cell line by upregulating the expression of target antioxidant proteins. While (+)-DHMEQ reduces the cytotoxic effect of 6-OHDA, its effect is less pronounced in Nrf2-knockdown cells produced with siRNA. ROS-generating 6-hydroxydopamine hydrochloride (6-OHDA) promotes the death of SK-N-SH cells. Consequently, Nrf2 activation is the reason behind (+)-DHMEQ's increased brain cell viability[1].
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References |
[1]. Niitsu Y, et al. Chemoenzymatic synthesis of (2R,3R,4R)-dehydroxymethylepoxyquinomicin (DHMEQ), a newactivator of antioxidant transcription factor Nrf2. Org Biomol Chem. 2011 Jun 21;9(12):4635-41
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Molecular Formula |
C13H11NO5
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Molecular Weight |
261.2301
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CAS # |
287194-41-6
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SMILES |
O1[C@@]2([H])C(C([H])=C([C@]([H])([C@@]12[H])O[H])N([H])C(C1=C([H])C([H])=C([H])C([H])=C1O[H])=O)=O
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~100 mg/mL (~382.80 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (9.57 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.8280 mL | 19.1402 mL | 38.2804 mL | |
5 mM | 0.7656 mL | 3.8280 mL | 7.6561 mL | |
10 mM | 0.3828 mL | 1.9140 mL | 3.8280 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.