| Size | Price | Stock | Qty |
|---|---|---|---|
| 5g |
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| Other Sizes |
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| ln Vitro |
An SS bond between the protein and the thionitrobenzoic acid (TNB) residue is created when DTNB combines with the tyrosyl side chain of cysteine [1]. The selectivity of this reagent (imine or iodoacetamide) and its capacity to track the reaction process spectrophotometrically are its key features [1]. Use DTNB to alter the enzyme: Stir 0.6 mL of 80 mM phosphate buffer (0.807 mg/mL) with 10 μL of 10 mM DTNB solution (about 20 times molar excess) to modify the SH group of the enzyme. A pH 8.0 enzyme solution with 1 mM EDTA (0.807 mg/mL) has been utilized in the past. Molar extinction coefficient of the ions produced by 13,600 M thionitrobenzoate In the tip, increase the number of SH groups. Give the same solution a 24-hour buffer.
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|---|---|
| References | |
| Additional Infomation |
Dithionitrobenzoic acid is an organic disulfide that results from the formal oxidative dimerisation of 2-nitro-5-thiobenzoic acid. An indicator used to quantify the number or concentration of thiol groups. It has a role as an indicator. It is a nitrobenzoic acid and an organic disulfide.
A standard reagent for the determination of reactive sulfhydryl groups by absorbance measurements. It is used primarily for the determination of sulfhydryl and disulfide groups in proteins. The color produced is due to the formation of a thio anion, 3-carboxyl-4-nitrothiophenolate. |
| Molecular Formula |
C14H8N2O8S2
|
|---|---|
| Molecular Weight |
396.34
|
| Exact Mass |
395.972
|
| CAS # |
69-78-3
|
| PubChem CID |
6254
|
| Appearance |
Off-white to yellow solid powder
|
| Density |
1.8±0.1 g/cm3
|
| Boiling Point |
671.9±55.0 °C at 760 mmHg
|
| Melting Point |
240-245 °C (dec.)(lit.)
|
| Flash Point |
360.1±31.5 °C
|
| Vapour Pressure |
0.0±2.2 mmHg at 25°C
|
| Index of Refraction |
1.769
|
| LogP |
3.97
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
10
|
| Rotatable Bond Count |
5
|
| Heavy Atom Count |
26
|
| Complexity |
528
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
KIUMMUBSPKGMOY-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)
|
| Chemical Name |
5-[(3-carboxy-4-nitrophenyl)disulfanyl]-2-nitrobenzoic acid
|
| Synonyms |
DTNB; DTNB; DTNB
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~252.30 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (5.25 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (5.25 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (5.25 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.5231 mL | 12.6154 mL | 25.2309 mL | |
| 5 mM | 0.5046 mL | 2.5231 mL | 5.0462 mL | |
| 10 mM | 0.2523 mL | 1.2615 mL | 2.5231 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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