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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg |
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Echinocystic acid is a naturally occurring pentacyclic triterpene found in the fruits of Gleditsia sinensis Lam with antioxidant, anti-inflammatory and anticancer pactivities.
| ln Vitro |
Echinocystic acid (2.5–10 μM; 1 hour off) suppresses the activation of NF-κB and MAPK caused by IL-1β [1]. Echinacetic acid exhibits dose-dependent inhibition of MMP-13, NO, and PGE2 produced by IL-1β. COX-2 and iNOS are upregulated in response to IL-1β, and echinocystic acid inhibits this upregulation [1].
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|---|---|
| ln Vivo |
In a dyad model of pain and depression generated by reserpine, echinocystic acid (5 mg/kg daily for 5 days) had antidepressant effects [2].
|
| Cell Assay |
Western Blot Analysis [1]
Cell Types: Chondrocytes Tested Concentrations: 2.5 μM, 5 μM, 10 μM Incubation Duration: Pretreatment for 1 hour, then stimulated with IL-1β for 30 minutes. Experimental Results: IL-1β-induced NF-κB and MAPK activation was inhibited. |
| Animal Protocol |
Animal/Disease Models: Male adult C57BL/6 mice (8-10 weeks old; 18 to 22 g) with reserpine injection [2]
Doses: 5 mg/kg Route of Administration: Intragastric; one time/day for 5 days Experimental Results: Partially alleviates the pain/depression binary effects of reserpine by modulating biogenic amine levels and GluN2B receptors in the hippocampus. |
| References | |
| Additional Infomation |
Echinocystic acid is a triterpenoid.
Echinocystic acid has been reported in Gleditsia sinensis, Eclipta alba, and other organisms with data available. |
| Molecular Formula |
C30H48O4
|
|---|---|
| Molecular Weight |
472.6997
|
| Exact Mass |
472.355
|
| CAS # |
510-30-5
|
| PubChem CID |
73309
|
| Appearance |
White to off-white solid powder
|
| Density |
1.1±0.1 g/cm3
|
| Boiling Point |
585.0±50.0 °C at 760 mmHg
|
| Melting Point |
299-300ºC
|
| Flash Point |
321.6±26.6 °C
|
| Vapour Pressure |
0.0±3.7 mmHg at 25°C
|
| Index of Refraction |
1.568
|
| LogP |
7.58
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
1
|
| Heavy Atom Count |
34
|
| Complexity |
919
|
| Defined Atom Stereocenter Count |
9
|
| SMILES |
C[C@]12CC[C@@H](C([C@@H]1CC[C@@]3([C@@H]2CC=C4[C@]3(C[C@H]([C@@]5([C@H]4CC(CC5)(C)C)C(=O)O)O)C)C)(C)C)O
|
| InChi Key |
YKOPWPOFWMYZJZ-PRIAQAIDSA-N
|
| InChi Code |
InChI=1S/C30H48O4/c1-25(2)14-15-30(24(33)34)19(16-25)18-8-9-21-27(5)12-11-22(31)26(3,4)20(27)10-13-28(21,6)29(18,7)17-23(30)32/h8,19-23,31-32H,9-17H2,1-7H3,(H,33,34)/t19-,20-,21+,22-,23+,27-,28+,29+,30+/m0/s1
|
| Chemical Name |
(4aR,5R,6aR,6aS,6bR,8aR,10S,12aR,14bS)-5,10-dihydroxy-2,2,6a,6b,9,9,12a-heptamethyl-1,3,4,5,6,6a,7,8,8a,10,11,12,13,14b-tetradecahydropicene-4a-carboxylic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~105.78 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.75 mg/mL (5.82 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 27.5 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1155 mL | 10.5775 mL | 21.1551 mL | |
| 5 mM | 0.4231 mL | 2.1155 mL | 4.2310 mL | |
| 10 mM | 0.2116 mL | 1.0578 mL | 2.1155 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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