Size | Price | Stock | Qty |
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250mg |
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500mg |
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1g |
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Other Sizes |
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Purity: ≥98%
Emodin (HSDB-7093; NSC-408120; NSC-622947; Emodol; Frangula emodin), a naturally occuring anthraquinone analog found in rubarb, buckthorn, and Japanese knotweed, is a potent and broad-spectrum anticancer agent that also has anti-inflammatory and neuroprotective effects. It inhibits casein kinase II (CKII) activity with IC50 of 2 μM.
ln Vitro |
Emodin (10–400 μM) has an IC50 value of 200 μM and inhibits S protein binding to ACE2 in a dose-dependent manner[1]. In a dose-dependent manner, emodin (5-50 μM) decreases the infectivity of S-protein pseudotyped retroviruses. The SARS-CoV S protein cannot attach to Vero E6 cells when imodin is present [1]. Emodin inhibits casein kinase 2 (CK2) at ATP concentrations of 50 μM, 30.0 μM, and 7.1 μM for CK2α wild type, Ile174Ala mutant, and His160Ala mutant, respectively, with an IC50 of 5.9. At ATP concentration, the IC50 values for the CK2α wild type and the Val66Ala mutant are 1.40 and 38.00 μM, respectively [2]. Emodin is more than 5000 times more selective for the human and mouse 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2) enzyme than type 2 isoenzymes, as seen by its modest inhibitory action against both mouse and human 11β-HSD2 (IC50 greater than 1 mM) [3].
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ln Vivo |
In normal C57BL/6J male mice, emodin (single oral treatment of 100 or 200 mg/kg) suppresses 11β-HSD1 activity [3]. Emodin (100 mg/kg; oral; bid) lowers blood glucose, liver PEPCK, and glucose-6-phosphatase mRNA in diet-induced obesity (DIO) rats, improving insulin sensitivity and lipid metabolism [3].
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Cell Assay |
Cell Viability Assay[1]
Cell Types: Vero E6 cells transfected with the plasmid encoding ACE2 Tested Concentrations: 0, 5, 25, 50 μM Incubation Duration: 24 hrs (hours) Experimental Results: Vero cells treated with 50 μM remained 82.4±3.8% viability, the anti -SARS-CoV activity was not due to toxicity. |
Animal Protocol |
Animal/Disease Models: C57BL/6J male mice[3]
Doses: 100 or 200 mg/kg Route of Administration: Acute administered po; Two hrs (hours) later, the mice were killed by cervical dislocation, Experimental Results: Dramatically inhibited liver 11β-HSD1 enzymatic activity by 17.6 and 31.3% and mesenteric fat 11β-HSD1 enzymatic activity by 21.5 and 46.7% at 100 or 200 mg/kg, respectively. Animal/Disease Models: DIO mice (C57BL/6J male mice were fed a formulated research diet)[3] Doses: 100 mg /kg Route of Administration: po (oral gavage); twice per day; for 35 days Experimental Results: decreased fasting glucose concentrations to 77.2% of the vehicle control mice after 7 days of treatment, and these remained Dramatically lower throughout the treatment period. demonstrated a significant reduction in blood glucose levels at all time-points following oral glucose challenge after 24 days of treatment. Evoked a Dramatically greater reduction in blood glucose values 40 and 90 min after insulin injection after 28 days of treatment. The serum insulin level was also significan |
References |
[1]. Tin-Yun Ho, et al. Emodin blocks the SARS coronavirus spike protein and angiotensin-converting enzyme 2 interaction. Antiviral Res. 2007 May;74(2):92-101.
[2]. Stefania Sarno, et al. Toward the rational design of protein kinase casein kinase-2 inhibitors. Pharmacol Ther. Feb-Mar 2002;93(2-3):159-68. [3]. Ying Feng, et al. Emodin, a natural product, selectively inhibits 11beta-hydroxysteroid dehydrogenase type 1 and ameliorates metabolic disorder in diet-induced obese mice. Br J Pharmacol. 2010 Sep;161(1):113-26. |
Molecular Formula |
C15H10O5
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Molecular Weight |
270.24
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CAS # |
518-82-1
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Related CAS # |
Emodin-d4;132796-52-2
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
O([H])C1=C([H])C(C([H])([H])[H])=C([H])C2C(C3C([H])=C(C([H])=C(C=3C(C=21)=O)O[H])O[H])=O
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InChi Key |
RHMXXJGYXNZAPX-UHFFFAOYSA-N
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InChi Code |
InChI=1S/C15H10O5/c1-6-2-8-12(10(17)3-6)15(20)13-9(14(8)19)4-7(16)5-11(13)18/h2-5,16-18H,1H3
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Chemical Name |
1,3,8-trihydroxy-6-methylanthracene-9,10-dione
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Synonyms |
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
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Solubility (In Vivo) |
Solubility in Formulation 1: 3.33 mg/mL (12.32 mM) in 0.5% CMC-Na/saline water (add these co-solvents sequentially from left to right, and one by one),Suspened solution; with sonication.
Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 10 mg/mL (37.00 mM) in 0.5% MC 0.5% Tween-80 (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.7004 mL | 18.5021 mL | 37.0041 mL | |
5 mM | 0.7401 mL | 3.7004 mL | 7.4008 mL | |
10 mM | 0.3700 mL | 1.8502 mL | 3.7004 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
NCT Number | Recruitment | interventions | Conditions | Sponsor/Collaborators | Start Date | Phases |
NCT00801268 | Terminated | Drug: tripterygium wilfordii Drug: Emodin |
Polycystic Kidney | Zhi-Hong Liu, M.D. | November 2008 | Not Applicable |