| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| Other Sizes |
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| Targets |
Nrf2
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| ln Vitro |
Eriodictyol is a flavonoid that has been isolated from the Chinese herb and exhibits anti-inflammatory and antioxidant properties[1]. Through the Nrf2/ARE pathway, eriodictyol (20, 40, and 80 μM) shields primary cultured cortical neurons from Aβ25-35-induced cytotoxicity and cell apoptosis. In primary cultured cortical neurons, eriodictyol (80 μM) induces nuclear expression of Nrf2 and the expression of ARE-regulated genes[2].
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| ln Vivo |
Eriodictyol exhibits a protective effect against acute lung injury (ALI) caused by lipotoxic shock. In LPS-induced ALI mice, eriodictyol (30 mg/kg, p.o.) reduces oxidative stress levels, increases survival rate, and reduces inflammatory cytokine production. MPO activity and inflammatory neutrophil accumulation in lung tissues are both inhibited by eriodictyol. By upregulating the expression of Nrf2 in lung tissues, eriodictyol treatment also increases the expression of Trx1[1].
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| Cell Assay |
Primary cultures of cortical neurons are generated from Sprague-Dawley rat embryos on day 17, and they are kept in Neurobasal A medium with B27 added. Seven days after seeding, experiments are conducted. To encourage aggregation, Aβ25-35 is dissolved in 1 mM deionized distilled water and incubated for 7 days at 37°C. The indicated concentrations of eriodictyol (20, 40, and 80 μM) are added two hours before Aβ25-35 treatment. Before being used, eriditol is diluted in culture medium after being dissolved in dimethyl sulfoxide (DMSO) at a concentration of 64 mM as a stock solution[2].
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| Animal Protocol |
Mice: One intraperitoneal injection each of 150 mg/kg ketamine HCl and 65 µg/kg xylazine hydrochloride is used to anesthetize 80 female C57BL/6 mice. During inspiration, 25 µg of E. coli LPS are injected intratracheally into 50 µL of sterile saline. Two days prior to the induction of ALI, the mice treated with Eriodictyol (30 mg/kg, dissolved in PBS) and the vehicle (PBS) receive oral administration, while the control mice receive PBS instillation. 24 hours after ALI induction, the mice are then killed by an intravenous injection of thiopental. A cardiac puncture is used to obtain a blood sample after the thorax is opened. Three bronchoalveolar lavage (BAL) operations are carried out concurrently, with 0.5 mL of normal saline used for each procedure. After centrifuging the blood (2,000 × g) for 10 minutes at 4°C, the serum is kept for later use. The Kaplan-Meier method is then used to illustrate the survival curve[1].
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| References |
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| Molecular Formula |
C₁₅H₁₂O₆
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|---|---|
| Molecular Weight |
288.2522
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| Exact Mass |
288.06
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| Elemental Analysis |
C, 62.50; H, 4.20; O, 33.30
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| CAS # |
552-58-9
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| Related CAS # |
(±)-Eriodictyol;4049-38-1
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| Appearance |
Solid powder
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| SMILES |
C1[C@H](OC2=CC(=CC(=C2C1=O)O)O)C3=CC(=C(C=C3)O)O
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| InChi Key |
SBHXYTNGIZCORC-ZDUSSCGKSA-N
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| InChi Code |
InChI=1S/C15H12O6/c16-8-4-11(19)15-12(20)6-13(21-14(15)5-8)7-1-2-9(17)10(18)3-7/h1-5,13,16-19H,6H2/t13-/m0/s1
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| Chemical Name |
(2S)-2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-2,3-dihydrochromen-4-one
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| Synonyms |
Huazhongilexone; 2S-Eriodictyol; (+)-Eriodictyol; (+) Eriodictyol; 2S Eriodictyol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~125 mg/mL (~433.7 mM)
H2O: ~0.1 mg/mL (~0.4 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.58 mg/mL (8.95 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.58 mg/mL (8.95 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.58 mg/mL (8.95 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.4692 mL | 17.3461 mL | 34.6921 mL | |
| 5 mM | 0.6938 mL | 3.4692 mL | 6.9384 mL | |
| 10 mM | 0.3469 mL | 1.7346 mL | 3.4692 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
| NCT Number | Recruitment | interventions | Conditions | Sponsor/Collaborators | Start Date | Phases |
| NCT03215043 | Completed | Dietary Supplement: Eriocitrin Other: Placebo |
Pre Diabetes | Thais Cesar | July 2, 2017 | Not Applicable |
| NCT03928249 | Completed | Dietary Supplement: Eriocitrin | Pre Diabetes | São Paulo State University | December 1, 2019 | Not Applicable |
| NCT03925909 | Terminated | Dietary Supplement: Eriocitrin | Pre Diabetes | São Paulo State University | May 1, 2019 | Not Applicable |
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