| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| Targets |
ER
|
|---|---|
| ln Vitro |
Human endometrial stem cells (hEnSC) are exposed to estradiol hemihydrate (10 nM) for seven days, during which time it promotes neurite branching and neural development [1]. In hEnSC-differentiated neuronal-like cells, estradiol hemihydrate (17β-estradiol, 10 nM, 7 days) enhances the expression of neuron-like cell markers (Tuj-1, nestin, and NF-H) [1]. Assay for cell differentiation [1]
|
| ln Vivo |
Estradiol hemihydrate (1 nM, hippocampal slices from FBN-ARO-KO mice) recovers long-term potentiation (LTP) amplitude [1]. Estradiol hemihydrate (0.0167 mg, subcutaneously implanted in FBN-ARO-KO mice) cures molecular and functional abnormalities in FBN-ARO-KO animals [1].
|
| Cell Assay |
Cell differentiation assay[1]
Cell Types: Human endometrial stem cells (hEnSC) isolated from human endometrial tissue Tested Concentrations: 10 nM Incubation Duration: 7 days Experimental Results: Neural markers (Tuj-1, Nestin and NF The percentage increases of -H)-positive cells were 62.2±1.3%, 71.5±4% and 51.2±1.5%, respectively. Immunofluorescence[1] Cell Types: Human endometrial stem cells (hEnSC) isolated from human endometrial tissue Tested Concentrations: 10 nM Incubation Duration: 7 days Experimental Results: Neural markers (Tuj-1, Nestin and NF- H) The percentage increases of positive cells were 62.2±1.3%, 71.5±4% and 51.2±1.5% respectively. |
| Animal Protocol |
Animal/Disease Models: FBN-ARO-KO mice [2]
Doses: 1 nM Route of Administration: Treatment of hippocampal slices Experimental Results: Long-term potentiation (LTP) amplitude was restored in both male and female mice. Animal/Disease Models: FBN-ARO-KO mice [2] Doses: 0.0167 mg Route of Administration: Alzet minipump containing estradiol (subcutaneously (sc) (sc) implanted) was used, and examinations were performed 7 days after minipump implantation. Experimental Results: E2 levels in the hippocampus and cortex were restored to 93%, AKT, ERK and CREB phosphorylation in the hippocampus and cortex were restored to 90-95%, BDNF levels were restored to 80-90%, and forebrain synaptophysin and PSD95 were restored. Rescues spatial learning and memory deficits. |
| References | |
| Additional Infomation |
Estradiol Hemihydrate is the hemihydrate form of estradiol, the most potent, naturally produced estrogen. Estradiol hemihydrate diffuses through the cell membrane and binds to and subsequently activates the nuclear estrogen receptor found in the reproductive tract, breast, pituitary, hypothalamus, liver, and bone. The activated complex binds to the estrogen response element on the DNA and activates the transcription of genes involved in the functioning of the female reproductive system and secondary sex characteristics.
The 17-beta-isomer of estradiol, an aromatized C18 steroid with hydroxyl group at 3-beta- and 17-beta-position. Estradiol-17-beta is the most potent form of mammalian estrogenic steroids. See also: Estradiol (has active moiety) ... View More ... |
| Molecular Formula |
2[C18H24O2].H2O
|
|---|---|
| Exact Mass |
562.365
|
| CAS # |
35380-71-3
|
| Related CAS # |
Alpha-Estradiol;57-91-0;Estradiol (Standard);50-28-2;Estradiol-d3;79037-37-9;Estradiol-d4;66789-03-5;Estradiol-d5;221093-45-4;Estradiol-13C2;82938-05-4;Estradiol (cypionate);313-06-4;Estradiol benzoate;50-50-0;Estradiol enanthate;4956-37-0;Estradiol hemihydrate;35380-71-3;Estradiol-d2;53866-33-4;Estradiol-13C6;Estradiol-d2-1;3188-46-3;rel-Estradiol-13C6; 979-32-8 (valerate); 113-38-2 (dipropionate); 57-63-6 (ethinyl); 172377-52-5 (sulfamate); 3571-53-7 (undecylate)
|
| PubChem CID |
154274
|
| Appearance |
Typically exists as solid at room temperature
|
| Boiling Point |
445.9ºC at 760mmHg
|
| Flash Point |
209.6ºC
|
| LogP |
3.544
|
| Hydrogen Bond Donor Count |
5
|
| Hydrogen Bond Acceptor Count |
5
|
| Rotatable Bond Count |
0
|
| Heavy Atom Count |
41
|
| Complexity |
382
|
| Defined Atom Stereocenter Count |
10
|
| SMILES |
O.OC1C=CC2[C@H]3CC[C@@]4([C@H](CC[C@H]4[C@@H]3CCC=2C=1)O)C.OC1C=CC2[C@H]3CC[C@@]4([C@H](CC[C@H]4[C@@H]3CCC=2C=1)O)C
|
| InChi Key |
ZVVGLAMWAQMPDR-WVEWYJOQSA-N
|
| InChi Code |
InChI=1S/2C18H24O2.H2O/c2*1-18-9-8-14-13-5-3-12(19)10-11(13)2-4-15(14)16(18)6-7-17(18)20;/h2*3,5,10,14-17,19-20H,2,4,6-9H2,1H3;1H2/t2*14-,15-,16+,17+,18+;/m11./s1
|
| Chemical Name |
(8R,9S,13S,14S,17S)-13-methyl-6,7,8,9,11,12,14,15,16,17-decahydrocyclopenta[a]phenanthrene-3,17-diol;hydrate
|
| Synonyms |
ESTRADIOL HEMIHYDRATE; 35380-71-3; beta-Estradiol semihydrate; UNII-CXY7B3Q98Z; CXY7B3Q98Z; Estra-1,3,5(10)-triene-3,17beta-diol hydrate (2:1); beta-Estradiol hemihydrate; Estrasorb (TN);
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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