Size | Price | Stock | Qty |
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5mg |
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10mg |
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25mg |
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50mg |
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Other Sizes |
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ln Vitro |
When used in conjunction with the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX), etymone (EN) significantly increased the production of testosterone in a dose-dependent manner at doses of 0.1, 1.0, and 10.0 μM; however, testosterone production at the two lower doses was not significantly higher than when IBMX or EN were used alone, except at higher concentrations. Molecular docking analyses revealed that the enzyme's binding sites for IBMX and EN are distinct. The inhibitory effects of nifedipine, ketoconazole, or aminoglutethimide on the respective steroid oogenesis enzymes are not reversed by EN. Quassamine likewise did not respond to tamoxifen's suppression of estrogen receptors, but it did better when mestane inhibition of aromatase was used to lower the production of estrogen. The fact that EN and formestane bind to aromatase with comparable orientations and free energy binding values is further supported by molecular docking studies [1].
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References |
[1]. Low BS, et al. Eurycomanone, the major quassinoid in Eurycoma longifolia root extract increases spermatogenesis by inhibiting the activity of phosphodiesterase and aromatase in steroidogenesis. J Ethnopharmacol. 2013 Aug 26;149(1):201-7.
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Molecular Formula |
C₂₀H₂₄O₉
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Molecular Weight |
408.40
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CAS # |
84633-29-4
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
O[C@@]1([]2O)[C@@]34[C@@]([C@](OC4)(O)[](O)C1=C)([H])[C@]([C@@](C(C)=CC5=O)([H])C[C@@]3([H])OC2=O)([C@@H]5O)C
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~16.67 mg/mL (~40.82 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 0.83 mg/mL (2.03 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 8.3 mg/mL clear DMSO stock solution to 400 μL of PEG300 and mix evenly; then add 50 μL of Tween-80 to the above solution and mix evenly; then add 450 μL of normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 0.83 mg/mL (2.03 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 8.3 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 0.83 mg/mL (2.03 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.4486 mL | 12.2429 mL | 24.4858 mL | |
5 mM | 0.4897 mL | 2.4486 mL | 4.8972 mL | |
10 mM | 0.2449 mL | 1.2243 mL | 2.4486 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.