Exo1

Alias: Exo1; Exo 1; Exo-1
Cat No.:V4001 Purity: ≥98%
Exo-1 (Exo1)is a novel, potent and reversible inhibitor of exocytosis which is used as a chemical inhibitor of theexocytic pathway.
Exo1 Chemical Structure CAS No.: 75541-83-2
Product category: Ras
This product is for research use only, not for human use. We do not sell to patients.
Size Price Stock Qty
5mg
10mg
25mg
50mg
100mg
250mg
Other Sizes
Official Supplier of:
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text

 

  • Business Relationship with 5000+ Clients Globally
  • Major Universities, Research Institutions, Biotech & Pharma
  • Citations by Top Journals: Nature, Cell, Science, etc.
Top Publications Citing lnvivochem Products
Purity & Quality Control Documentation

Purity: ≥98%

Product Description

Exo-1 (Exo1) is a novel, potent and reversible inhibitor of exocytosis which is used as a chemical inhibitor of the exocytic pathway. Golgi ARF 1 (ADP-Ribosylation Factor) GTPase is activated as a result of it. Exo1 triggers a swift collapse of the Golgi apparatus to the endoplasmic reticulum, thereby sharply impeding the traffic that emerges from the latter. Exo1 causes ADP-ribosylation factor (ARF) 1 to be released from Golgi membranes quickly, much like Brefeldin A (BFA), but it has less of an impact on how the trans-Golgi network is organized. Exo1 functions via a distinct pathway than BFA. Exo1 does not, in contrast to BFA, cause CtBP/Bars50 to become ADP-ribosylated or obstruct the function of guanine nucleotide exchange factors that are particular to Golgi-based ARFs. Consequently, Exo1 permits the separation of Bars50's fatty acid exchange activity from ARF1 activity in the regulation of Golgi tubulation.

Biological Activity I Assay Protocols (From Reference)
Targets
exocytosis; Golgi; ARF-1
ln Vitro
Exo1 modifies the GTPase activity of Golgi ARF1. Exo1 offers a separate method of interfering with Golgi activity because it blocks a portion of the membrane events that BFA blocks, although it appears to target a different protein target and likely has different side effects. Exo1 Disrupts Golgi structures to disrupt vesicular traffic from the ER to the Golgi apparatus. Exo1 has an IC50 of about 20 μM, which inhibits exocytosis. Exo1 inhibits newly synthesized proteins like VSVGts-GFP, transferrin, and MHC class I from acquiring endoglycosidase H resistance[1].
References

[1]. Exo1: a new chemical inhibitor of the exocytic pathway. Proc Natl Acad Sci U S A. 2003 May 27;100(11):6469-74.

These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C15H12FNO3
Molecular Weight
273.26
Exact Mass
273.08
Elemental Analysis
C, 65.93; H, 4.43; F, 6.95; N, 5.13; O, 17.56
CAS #
75541-83-2
Appearance
Solid powder
SMILES
COC(=O)C1=CC=CC=C1NC(=O)C2=CC=C(C=C2)F
InChi Key
KIAPWMKFHIKQOZ-UHFFFAOYSA-N
InChi Code
InChI=1S/C15H12FNO3/c1-20-15(19)12-4-2-3-5-13(12)17-14(18)10-6-8-11(16)9-7-10/h2-9H,1H3,(H,17,18)
Chemical Name
methyl 2-[(4-fluorobenzoyl)amino]benzoate
Synonyms
Exo1; Exo 1; Exo-1
HS Tariff Code
2934.99.9001
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO: ~10mM
Water: N/A
Ethanol: N/A
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 3.6595 mL 18.2976 mL 36.5952 mL
5 mM 0.7319 mL 3.6595 mL 7.3190 mL
10 mM 0.3660 mL 1.8298 mL 3.6595 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

Calculator

Molarity Calculator allows you to calculate the mass, volume, and/or concentration required for a solution, as detailed below:

  • Calculate the Mass of a compound required to prepare a solution of known volume and concentration
  • Calculate the Volume of solution required to dissolve a compound of known mass to a desired concentration
  • Calculate the Concentration of a solution resulting from a known mass of compound in a specific volume
An example of molarity calculation using the molarity calculator is shown below:
What is the mass of compound required to make a 10 mM stock solution in 5 ml of DMSO given that the molecular weight of the compound is 350.26 g/mol?
  • Enter 350.26 in the Molecular Weight (MW) box
  • Enter 10 in the Concentration box and choose the correct unit (mM)
  • Enter 5 in the Volume box and choose the correct unit (mL)
  • Click the “Calculate” button
  • The answer of 17.513 mg appears in the Mass box. In a similar way, you may calculate the volume and concentration.

Dilution Calculator allows you to calculate how to dilute a stock solution of known concentrations. For example, you may Enter C1, C2 & V2 to calculate V1, as detailed below:

What volume of a given 10 mM stock solution is required to make 25 ml of a 25 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=25 μM, V2=25 ml and V1 is the unknown:
  • Enter 10 into the Concentration (Start) box and choose the correct unit (mM)
  • Enter 25 into the Concentration (End) box and select the correct unit (mM)
  • Enter 25 into the Volume (End) box and choose the correct unit (mL)
  • Click the “Calculate” button
  • The answer of 62.5 μL (0.1 ml) appears in the Volume (Start) box
g/mol

Molecular Weight Calculator allows you to calculate the molar mass and elemental composition of a compound, as detailed below:

Note: Chemical formula is case sensitive: C12H18N3O4  c12h18n3o4
Instructions to calculate molar mass (molecular weight) of a chemical compound:
  • To calculate molar mass of a chemical compound, please enter the chemical/molecular formula and click the “Calculate’ button.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
  • Molecular mass (or molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
  • Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
/

Reconstitution Calculator allows you to calculate the volume of solvent required to reconstitute your vial.

  • Enter the mass of the reagent and the desired reconstitution concentration as well as the correct units
  • Click the “Calculate” button
  • The answer appears in the Volume (to add to vial) box
In vivo Formulation Calculator (Clear solution)
Step 1: Enter information below (Recommended: An additional animal to make allowance for loss during the experiment)
Step 2: Enter in vivo formulation (This is only a calculator, not the exact formulation for a specific product. Please contact us first if there is no in vivo formulation in the solubility section.)
+
+
+

Calculation results

Working concentration mg/mL;

Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
             (2) Be sure to add the solvent(s) in order.

Clinical Trial Information
NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04491240 Completed Drug: EXO 1 inhalation
Drug: EXO 2 inhalation
Covid19
COVID-19
State-Financed Health Facility
"Samara Regional Medical Center Dinasty"
July 20, 2020 Phase 1
Phase 2
Biological Data
  • Exo1

    Exit of VSVGts-GFP from the ER is inhibited by Exo1.


    Exo1

    Exo1 does not induce the ADP-ribosylation of Bars50.2003 May 27;100(11):6469-74.

  • Exo1

    Exo1 induces tubulation and collapse of the Golgi apparatus but not endosomes and TGN.

  • Exo1

    Exo1 acts on ARF1-GTPase through a different mechanism compared with BFA.2003 May 27;100(11):6469-74.

Contact Us