| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| Other Sizes |
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| ln Vitro |
With IC50 values of 19.0 µM (24 hours), 12.0 µM (48 hours), and 7.57 µM (5637 72 hours), 11.9 µM (24 hours), 9.92 µM (48 hours), and 7.13 µM (72 hours) in cells, fumangchinoline (2.5–40 µM; 24-96 hours) inhibits T24 and 5637 cells in a dose-dependent manner [1]. When applied to T24 and 5637 cells, fanchinoline (5 µM; 24 hours) significantly increases caspase-3 cleavage, decreases p62, and increases the ratio of LC3-II/LC3-I [1].
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|---|---|
| Cell Assay |
Cell Viability Assay[3]
Cell Types: T24 and 5637 Cell Tested Concentrations: 2.5 µM; 5 µM; 10 µM; 20 µM; 30 µM; 40 µM Incubation Duration: 24 hrs (hours); 48 hrs (hours); 96 hrs (hours) Experimental Results: Inhibition of T24 and 5637 cell proliferation . Western Blot Analysis[3] Cell Types: T24 and 5637 Cell Tested Concentrations: 5 µM Incubation Duration: 24 hrs (hours) Experimental Results: Increased LC3-II/LC3-I ratio and caspase-3 cleavage. |
| References |
[1]. Wan Z, et al. Fangchinoline inhibits human immunodeficiency virus type 1 replication by interfering with gp160 proteolytic processing. PLoS One. 2012;7(6):e39225.
[2]. Guo B, et al. Fangchinoline as a kinase inhibitor targets FAK and suppresses FAK-mediated signaling pathway in A549. J Drug Target. 2015 Apr;23(3):266-74. [3]. Fan B, et al. Fangchinoline Induces Apoptosis, Autophagy and Energetic Impairment in Bladder Cancer. Cell Physiol Biochem. 2017;43(3):1003-1011. |
| Molecular Formula |
C37H40N2O6
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|---|---|
| Molecular Weight |
608.7233
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| CAS # |
436-77-1
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| Related CAS # |
(R)-Fangchinoline;33889-68-8
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| Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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| SMILES |
O1C2=C(C([H])=C3C([H])([H])C([H])([H])N(C([H])([H])[H])[C@@]([H])(C([H])([H])C4C([H])=C([H])C(=C([H])C=4[H])OC4=C(C([H])=C([H])C(=C4[H])C([H])([H])[C@@]4([H])C5=C1C(=C(C([H])=C5C([H])([H])C([H])([H])N4C([H])([H])[H])OC([H])([H])[H])O[H])OC([H])([H])[H])C3=C2[H])OC([H])([H])[H]
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~82.14 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (3.42 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (3.42 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.6428 mL | 8.2140 mL | 16.4279 mL | |
| 5 mM | 0.3286 mL | 1.6428 mL | 3.2856 mL | |
| 10 mM | 0.1643 mL | 0.8214 mL | 1.6428 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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