Size | Price | |
---|---|---|
5mg | ||
10mg | ||
15mg | ||
25mg |
FD-1080 (FD1080) is a novel and potent fluorophore with both excitation and emission in the NIR-II region and thus can be used for in vivo imaging.
ln Vitro |
1. Creating the workable solution for FD-1080 1.1 Get the storage solution ready. 10 mM FD-1080 is created by diluting FD-1080 with DMSO. Note: It is advised to aliquot the FD-1080 storage solution and keep it at -20°C or -80°C in the dark. 1.2 Setting up a functional solution: To make a working solution for FD-1080 that ranges from 2 to 10 μM, dilute the stock solution with hot PBS. Use a 0.2 μm filter to filter and sterilize. Note: Please prepare the FD-1080 working fluid for usage by adjusting its concentration to suit the current circumstances. 2. Experiment with cells 2.1 Cells in suspension: Centrifuge the cells to collect them, then add PBS and wash twice for five minutes each time. Trypsin should be added in order to break down adherent cells after discarding the culture media. Centrifuge, remove supernatant, add PBS, and wash twice for five minutes each time. 2.2 Add 1 milliliter of FD-1080 working solution, then let it sit at room temperature for ten to thirty minutes. 2.3 Discard the supernatant after centrifuging at 400 g for three to four minutes at 4°C. 2.4 After adding PBS, wash the cells twice for five minutes each. 2.5 Re-suspend the cells in 1 milliliter of PBS or serum-free medium, and examine using a flow cytometer or fluorescence microscope. 3. Intracellular injection 3.1 Give the mouse an intravenous injection of 200 μL of an 80 μM FD-1080 working solution, and after 10–20 minutes, conduct an in vivo imaging analysis.
|
---|---|
ln Vivo |
Compared to NIR excitation from 650 nm to 980 nm, the 1064 nm NIR-II excitation of FD-1080 is shown to have a higher tissue penetration depth and better imaging resolution. It was possible to obtain deeptissue and high-resolution in vivo imaging that could penetrate through intact skin, tissue, and skull for the left hindlimb vasculature, abdomen, and brain vessels. For both awake and anesthetized mice, FD-1080 measures the respiratory rate via dynamic imaging of the liver's respiratory craniocaudal motion[1].
|
References |
[1]. Li B, et al. An Efficient 1064 nm NIR-II Excitation Fluorescent Molecular Dye for Deep-Tissue High-Resolution Dynamic Bioimaging. Angew Chem Int Ed Engl. 2018 Jun 18;57(25):7483-7487.
[2]. Benhao Li, et al. An Efficient 1064 nm NIR-II Excitation Fluorescent Molecular Dye for Deep-Tissue High-Resolution Dynamic Bioimaging. Angew Chem Int Ed Engl. 2018 Jun 18;57(25):7483-7487. |
Molecular Formula |
C40H38CLN2NAO6S2
|
---|---|
Molecular Weight |
765.312298297882
|
CAS # |
1151666-58-8
|
Related CAS # |
FD-1080 free acid;1151888-25-3
|
Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
|
SMILES |
ClC1/C(=C/C=C2\C3=CC=CC4=CC=CC(=C34)N\2CCCCS(=O)(=O)[O-])/CCCC=1C=CC1C2=CC=CC3=CC=CC(=C23)[N+]=1CCCCS(=O)(=O)[O-].[Na+] |t:33|
|
HS Tariff Code |
2934.99.9001
|
Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
---|---|
Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80: Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 :Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.3067 mL | 6.5333 mL | 13.0666 mL | |
5 mM | 0.2613 mL | 1.3067 mL | 2.6133 mL | |
10 mM | 0.1307 mL | 0.6533 mL | 1.3067 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.