| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg |
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Purity: ≥98%
FH1 (also known as NSC 12407, BRD-K4477) can enhance the functions of cultured hepatocytes by promoting differentiation of iPS-derived hepatocytes. FH1 belongs to the function-only hits which are screened out by their ability to permit renewable sourcing of functional human hepatocytes. Besides that, FH1 can promote differentiation of iPS-derived hepatocytes towards a more mature phenotype. It doubles albumin secretion during the differentiation of iPS cells into iHeps.
| ln Vitro |
FH1 (NSC 12407, 5 µg/mL, 0–40 days) promotes hepatic maturation and preserves the hiPSC–HLC condition of hepatic differentiation[2].
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| ln Vivo |
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| Cell Assay |
RT-PCR[2]
Cell Types: hiPSC-HLCs in the differentiated hepatic state. Tested Concentrations: 5 µg/mL. Incubation Duration: 0-40 days. Experimental Results: Kept more than 50% hiPSC-HLCs to produce the four factors till day 40. Can help hiPSC-HLCs to maintain a relatively mature hepatic differentiation state for about 40 days. Maintained a higher level of hALB in the supernatant than that in controls. Maintained relatively high mRNA levels of hNTCP, hRXR, hHNF4α, and hALB for over functional small molecule FH1 can help hiPSC-HLCs to maintain a relatively mature hepatic differentiation state for about 40 days. |
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| Animal Protocol |
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| References |
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| Molecular Formula |
C17H18N2O2
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| Molecular Weight |
282.34
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| Exact Mass |
282.136
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| Elemental Analysis |
C, 72.32; H, 6.43; N, 9.92; O, 11.33
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| CAS # |
2719-05-3
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| Related CAS # |
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| PubChem CID |
94990
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| Appearance |
Solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
564.6±43.0 °C at 760 mmHg
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| Flash Point |
217.8±28.3 °C
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| Vapour Pressure |
0.0±1.5 mmHg at 25°C
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| Index of Refraction |
1.635
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| LogP |
1.93
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
21
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| Complexity |
320
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O=C(C([H])([H])[H])N([H])C1C([H])=C([H])C(=C([H])C=1[H])C([H])([H])C1C([H])=C([H])C(=C([H])C=1[H])N([H])C(C([H])([H])[H])=O
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| InChi Key |
OEXMNSOPAKOPEF-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C17H18N2O2/c1-12(20)18-16-7-3-14(4-8-16)11-15-5-9-17(10-6-15)19-13(2)21/h3-10H,11H2,1-2H3,(H,18,20)(H,19,21)
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| Chemical Name |
N-[4-[(4-acetamidophenyl)methyl]phenyl]acetamide
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| Synonyms |
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (8.85 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.85 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (8.85 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.5418 mL | 17.7091 mL | 35.4183 mL | |
| 5 mM | 0.7084 mL | 3.5418 mL | 7.0837 mL | |
| 10 mM | 0.3542 mL | 1.7709 mL | 3.5418 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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