| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| ln Vitro |
Fusion protein technology has emerged as a crucial instrument for addressing various issues related to the production of recombinant proteins. The attached tag's nature makes it easier to identify the fusion protein and offers a one-step purification process by allowing cell extracts or supernatants to be passed through a column in the right matrix. The FLAG peptide is permitted to elute in nondenaturing circumstances. The FLAG peptide is the target of several antibodies that have been created. The peptide and one antibody, M1, bind when divalent metal cations are present, preferably Ca2+. Chelation agents facilitate elution. Using an excess of free FLAGe peptide during competitive elution is another tactic. In this process, antibodies M2 and M5 were employed [1]. Monoclonal antibody epitopes that are dependent on calcium were the first to be identified as flag-tags. It is an octapeptide with a high acidity that can have its N-terminus fused to the target protein. The Flag tag has a high surface probability because it is a highly hydrophilic peptide. Immunoaffinity columns can be used to collect flag fusion proteins when Ca2+ is present, and EDTA can be used to elute the proteins at low concentrations, neutral pH, and conditions that are close to physiological values [2].
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| References |
[1]. Einhauer A, et al. The FLAG peptide, a versatile fusion tag for the purification of recombinant proteins. J Biochem Biophys Methods. 2001 Oct 30;49(1-3):455-65.
[2]. Schuster M, et al. Protein expression in yeast; comparison of two expression strategies regarding proteinmaturation. J Biotechnol. 2000 Dec 28;84(3):237-48. |
| Molecular Formula |
C41H60N10O20
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|---|---|
| Molecular Weight |
1012.97011089325
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| CAS # |
98849-88-8
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| SMILES |
O=C([C@H](CCCCN([H])[H])N([H])C([C@H](CC1C=CC(=CC=1)O)N([H])C([C@H](CC(=O)O)N([H])[H])=O)=O)N([H])[C@H](C(N([H])[C@@H](CC(=O)O)C(N([H])[C@@H](CC(=O)O)C(N([H])[C@@H](CC(=O)O)C(N([H])[C@H](C(=O)O)CCCCN([H])[H])=O)=O)=O)=O)CC(=O)O
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~100 mg/mL (~98.72 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (98.72 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.9872 mL | 4.9360 mL | 9.8720 mL | |
| 5 mM | 0.1974 mL | 0.9872 mL | 1.9744 mL | |
| 10 mM | 0.0987 mL | 0.4936 mL | 0.9872 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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