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100mg | ||
250mg | ||
500mg | ||
1g |
Purity: ≥98%
Fosfomycin is a potent phosphoenolpyruvate analog and a synthetic broad-spectrum antibiotic with antimicrobial and bactericidal properties. Fosfomycin binds to and inactivates the enzyme enolpyruvate transferase. This leads to an irreversible blockage of the condensation of uridine diphosphate-N-acetylglucosamine with p-enolpyruvate, which is one of the first steps of bacterial cell wall synthesis .
Targets |
Bacterial cell wall synthesis
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ln Vitro |
Fosfomycin sodium is an antibacterial agent for epoxy. In contrast to other antibacterial drugs, its mechanism of action involves impeding the first stage of cell wall formation [1]. With a 90% inhibition rate, fosfomycin sodium exhibits bactericidal efficacy against a range of Gram-positive and Gram-negative pathogenic bacteria, including β-bacteria that produce carbapenemase and extended-spectrum lactamases[1]. Because of its wide tissue penetration, fosfomycin sodium is useful in the research of infections pertaining to the lungs, soft tissue, bone, central nervous system, and abscesses [2].
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ln Vivo |
In rats, fosfomycin (80 mg/kg; orally or intravenously) protects against double bekacin nephrotoxicity and is unaffected by administration routes [3]. Rats' fosfomycin pharmacokinetics [4] Dibekacin dosage (mg) Vdss (l/kg) β (min-1) T1/2 (min) Urinary recovery rate (%) 30 0.261 0.0244 28.4 85
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Enzyme Assay |
Fosfomycin is a bactericidal antibiotic agent. It inhibits an enzyme-catalyzed reaction in the first step of the synthesis of the bacterial cell wall. Fosfomycin interferes with the first cytoplasmic step of bacterial cell wall biosynthesis, the formation of the peptidoglycan precursor UDP N-acetylmuramic acid (UDP-MurNAc). Specifically, the enzyme UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) is involved in peptidoglycan biosynthesis by catalyzing the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 3′-hydroxyl group of UDP-N-acetylglucosamine (UNAG). Fosfomycin covalently binds to the thiol group of a cysteine (position 115 in Escherichia coli numbering; target Cys115) in the active site of MurA and consequently inactivates it. This inhibitory action takes place at an earlier step than the action of β-lactams or glycopeptides [1].
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Cell Assay |
Fosfomycin exerts immunomodulatory effects by altering lymphocyte, monocyte and neutrophil function. It affects the acute inflammatory cytokine response in vitro and in vivo. It suppresses production of tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β), and IL-1α and increases production of IL-10, while contradictory data have been published regarding IL-6. On the other hand, concentrations of TNF-α, IL-1β, and IL-6 expressed as protein and mRNA were almost identical with and without fosfomycin in healthy volunteers. Fosfomycin suppresses IL-2 production from T cells, the production of leukotriene B4 (LTB4) from neutrophils, and the expression of IL-8 mRNA by LTB4 from monocytes. Fosfomycin also exhibits an immunomodulatory effect on B-cell activation. Fosfomycin enhances neutrophil phagocytic killing of invading pathogens, even in patients on chronic hemodialysis and renal transplantation). Fosfomycin resulted in enhanced bactericidal ability of neutrophils compared to other antimicrobials. The clinical relevance of the aforementioned actions remains to be elucidated [1].
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Animal Protocol |
Animal/Disease Models: Fischer 344 rats [3]
Doses: 320 mg/kg Route of Administration: intramuscularinjection, 5 courses of treatment: 1 hour, 0.5 hrs (hrs (hours)) earlier than Dibekacin, at the same time, 0.5 hrs (hrs (hours)) later, later 1 hour; 11 days Experimental Results: Following previous treatment, polyuria, proteinuria, enzymes and cytosine were diminished due to dibekacin (40 mg/kg). Animal/Disease Models: Acute renal failure dehydrated Wistar rats (8 weeks old) [4] Doses: 120 mg/kg Route of Administration: intravenous (iv) (iv)injection; 200mg/kg. The first Experimental Results:the elimination rate of rats basically returned to normal, and the nephrotoxicity parameters improved. Protects proximal tubular lysosomes from the effects of aminoglycosides by inhibiting myelopoiesis and protecting the integrity of lysosomal membranes in rats treated with bibekacin. |
References |
[1]. Falagas ME, et al. Fosfomycin. Clin Microbiol Rev. 2016 Apr. 29(2):321-47.
[2]. Dijkmans AC, et al. Fosfomycin: Pharmacological, Clinical and Future Perspectives. Antibiotics (Basel). 2017 Oct 31. 6(4):24. [3]. Inouye S, et al. Mode of protective action of fosfomycin against dibekacin-induced nephrotoxicity in the dehydrated rats. J Pharmacobiodyn. 1982 Dec. 5(12):941-50. |
Molecular Formula |
C3H7O4P
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Molecular Weight |
138.05908
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Exact Mass |
138.008
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Elemental Analysis |
C, 26.10; H, 5.11; O, 46.35; P, 22.44
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CAS # |
23155-02-4
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Related CAS # |
Fosfomycin calcium;26016-98-8;Fosfomycin sodium;26016-99-9;(Rac)-Fosfomycin (benzylamine)-13C3;1216461-18-5
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Appearance |
Solid powder
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LogP |
-1.4
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tPSA |
79.87
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SMILES |
C[C@H]1[C@@H](P(O)(O)=O)O1
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InChi Key |
YMDXZJFXQJVXBF-STHAYSLISA-N
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InChi Code |
InChI=1S/C3H7O4P/c1-2-3(7-2)8(4,5)6/h2-3H,1H3,(H2,4,5,6)/t2-,3+/m0/s1
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Chemical Name |
((2R,3S)-3-methyloxiran-2-yl)phosphonic acid
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Synonyms |
MK-955 MK 955 MK955 Antibiotic 833A Fosfonomycin
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HS Tariff Code |
2934.99.03.00
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO: > 10 mM
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Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80: Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 :Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 7.2432 mL | 36.2161 mL | 72.4323 mL | |
5 mM | 1.4486 mL | 7.2432 mL | 14.4865 mL | |
10 mM | 0.7243 mL | 3.6216 mL | 7.2432 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.