| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| Other Sizes |
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| ln Vitro |
Two HNSCC cell lines (CAL27 and UMSCC1) are evaluated, and the growth of these lines is inhibited by garcinol (10-50 μM; 24-72 hours) in a time- and dose-dependent way [3]. In HNSCC cells, garcinol (10–50 μM; 24-72 hours) causes apoptosis [3]. In a time-dependent manner, garcinol (50 μM; 1-6 hours) suppresses the phosphorylation and degradation of constitutive IκBα [3].
|
|---|---|
| ln Vivo |
Garcinol (intraperitoneal injection; 1 and 2 mg/kg; 5 times per week for 4 weeks) effectively slows tumor development [3].
|
| Cell Assay |
Cell proliferation assay[3]
Cell Types: CAL27 and UMSCC1 Cell Tested Concentrations: 10, 25, 50 µM Incubation Duration: 24, 48 and 72 hrs (hours) Experimental Results: Inhibition of proliferation of both HNSCC cell lines in a time- and dose-dependent manner. Apoptosis analysis[3] Cell Types: CAL27 and UMSCC1 Cell Tested Concentrations: 10, 25, 50 µM Incubation Duration: 24, 48 and 72 hrs (hours) Experimental Results: Induction of apoptosis in HNSCC cells. Western Blot Analysis[3] Cell Types: CAL27 Cell Tested Concentrations: 50 µM Incubation Duration: 1, 2, 4, 6 hrs (hours) Experimental Results: Inhibition of constitutive IκBα phosphorylation and degradation in a time-dependent manner. |
| Animal Protocol |
Animal/Disease Models: Fiveweeks old athymic nu/nu male mice bearing subcutaneousCAL27 tumors [3]
Doses: 1 and 2 mg/kg Route of Administration: intraperitoneal (ip) injection; five times per week for 4 weeks Experimental Results: Significant Inhibit tumor growth. |
| References |
| Molecular Formula |
C38H50O6
|
|---|---|
| Molecular Weight |
602.8
|
| Exact Mass |
602.36
|
| CAS # |
78824-30-3
|
| PubChem CID |
5490884
|
| Appearance |
Light yellow to yellow solid powder
|
| Density |
1.1±0.1 g/cm3
|
| Boiling Point |
710.8±60.0 °C at 760 mmHg
|
| Melting Point |
235-236ºC
|
| Flash Point |
397.6±29.4 °C
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| Vapour Pressure |
0.0±2.4 mmHg at 25°C
|
| Index of Refraction |
1.563
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| LogP |
11.26
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| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
6
|
| Rotatable Bond Count |
10
|
| Heavy Atom Count |
44
|
| Complexity |
1300
|
| Defined Atom Stereocenter Count |
4
|
| SMILES |
CC(=CC[C@@H]1C[C@@]2(C(=O)/C(=C(/C3=CC(=C(C=C3)O)O)\O)/C(=O)[C@@](C2=O)(C1(C)C)CC=C(C)C)C[C@H](CC=C(C)C)C(=C)C)C
|
| InChi Key |
DTTONLKLWRTCAB-UDFURZHRSA-N
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| InChi Code |
InChI=1S/C38H50O6/c1-22(2)11-13-27(25(7)8)20-37-21-28(15-12-23(3)4)36(9,10)38(35(37)44,18-17-24(5)6)34(43)31(33(37)42)32(41)26-14-16-29(39)30(40)19-26/h11-12,14,16-17,19,27-28,39-41H,7,13,15,18,20-21H2,1-6,8-10H3/b32-31+/t27-,28+,37+,38-/m0/s1
|
| Chemical Name |
(1S,3E,5R,7R)-3-[(3,4-dihydroxyphenyl)-hydroxymethylidene]-6,6-dimethyl-5,7-bis(3-methylbut-2-enyl)-1-[(2S)-5-methyl-2-prop-1-en-2-ylhex-4-enyl]bicyclo[3.3.1]nonane-2,4,9-trione
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| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~82.95 mM)
Ethanol : ~20 mg/mL (~33.18 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (4.15 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.6589 mL | 8.2946 mL | 16.5893 mL | |
| 5 mM | 0.3318 mL | 1.6589 mL | 3.3179 mL | |
| 10 mM | 0.1659 mL | 0.8295 mL | 1.6589 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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