| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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GC7 Sulfate, the sulfate salt of GC-7 (N1-guanyl-1,7-diaminoheptane), is a novel and potent deoxyhypusine synthase (DHPS) inhibitor that inhibits Neuroblastoma (NB) cell proliferation in a dose-dependent manner, through induction of the cell cycle inhibitor p21 and reduction of total and phosphorylated Rb proteins.
| Targets |
DHPS; p21
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|---|---|
| ln Vitro |
GC7 Sulfate is a deoxyhypusine synthase (DHPS) inhibitor that exhibits the ability to suppress MYCN-amplified and chemotherapy-resistant NB cell proliferation. It also modulates multiple cell cycle-regulating proteins and initiates arrest through the p21/Cdk4/Rb signaling axis, even when MYCN is present.[1]
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| Cell Assay |
The MTS assay kit is used to assess cell viability in compliance with the manufacturer's instructions. To summarize, cells are treated with GC7 Sulfate in 96-well plates for 72 hours at different concentrations (0.1 to 100 μM). Following this, they are immediately incubated with the MTS dye reagent for 1 hour at 37°C in 5% CO2 atmosphere. Using a reader, the absorbance is measured at 490 nm.
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| References |
| Molecular Formula |
C8H22N4O4S
|
|---|---|
| Molecular Weight |
270.349680423737
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| Exact Mass |
270.14
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| Elemental Analysis |
C, 35.54; H, 8.20; N, 20.72; O, 23.67; S, 11.86
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| CAS # |
150417-90-6
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| Appearance |
Powder
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| SMILES |
C(CCCN)CCCN=C(N)N.OS(=O)(=O)O
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| InChi Key |
MDDOWYFCKAAANU-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C8H20N4.H2O4S/c9-6-4-2-1-3-5-7-12-8(10)11;1-5(2,3)4/h1-7,9H2,(H4,10,11,12);(H2,1,2,3,4)
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| Chemical Name |
2-(7-aminoheptyl)guanidine;sulfuric acid
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| Synonyms |
N1-Guanyl-1,7-diaminoheptane; GC7 (sulfate)
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
Water : 3 mg/mL
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 5 mg/mL (18.49 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication (<60°C).
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.6989 mL | 18.4945 mL | 36.9891 mL | |
| 5 mM | 0.7398 mL | 3.6989 mL | 7.3978 mL | |
| 10 mM | 0.3699 mL | 1.8495 mL | 3.6989 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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