| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| 5mg |
|
||
| 10mg |
|
||
| Other Sizes |
|
| ln Vitro |
One hour before and after chronic ethanol exposure, GET73 (1 µM, 10 µM; 1 hour) guards against ethanol-induced reductions in cell viability, cytoskeletal changes, lower extracellular glutamate levels, and enhanced reactive oxygen species formation [1].
|
|---|---|
| ln Vivo |
The elevated plus maze (EPM) test in Sprague-Dawley rats exposed to GET73 (5–50 mg/kg; ir) results in anxiolytic effects [3].
|
| Cell Assay |
Cell Viability Assay[1]
Cell Types: Rat Hippocampal Neurons Tested Concentrations: 0.1 µM, 1 µM, 10 µM Incubation Duration: 1 hour Experimental Results: Prevention of ethanol (75 mM, 4 days)-induced decrease in MTT. |
| Animal Protocol |
Animal/Disease Models: Male SD rat (200-300 g and 300-350 g) [3]
Doses: 5 mg/kg, 10 mg/kg, 25 mg/kg, 50 mg/kg Route of Administration: po (oral gavage) Experimental Results: Exerts anxiolytic effects in rats exposed to the elevated plus maze (EPM) test. |
| References |
[1]. Tomasini MC, et al. GET73 Prevents Ethanol-Induced Neurotoxicity in Primary Cultures of Rat Hippocampal Neurons. Alcohol Alcohol. 2016 Mar;51(2):128-35.
[2]. Ferraro L, et al. GET73 modulates rat hippocampal glutamate transmission: evidence for a functional interaction with mGluR5. Pharmacol Rep. 2011;63(6):1359-71. [3]. Loche A, et al. Anti-Alcohol and Anxiolytic Properties of a New Chemical Entity, GET73. Front Psychiatry. 2012 Feb 14;3:8. |
| Additional Infomation |
Get 73 has been investigated for the treatment of Alcohol Dependence.
|
| Molecular Formula |
C₁₃H₁₆F₃NO₂
|
|---|---|
| Molecular Weight |
275.27
|
| Exact Mass |
275.113
|
| CAS # |
202402-01-5
|
| PubChem CID |
10221200
|
| Appearance |
Typically exists as solid at room temperature
|
| LogP |
3.588
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
5
|
| Rotatable Bond Count |
6
|
| Heavy Atom Count |
19
|
| Complexity |
274
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
FC(C1C=CC(=CC=1)CNC(CCCOC)=O)(F)F
|
| InChi Key |
QLZOWJNFLXSDSH-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C13H16F3NO2/c1-19-8-2-3-12(18)17-9-10-4-6-11(7-5-10)13(14,15)16/h4-7H,2-3,8-9H2,1H3,(H,17,18)
|
| Chemical Name |
4-methoxy-N-[[4-(trifluoromethyl)phenyl]methyl]butanamide
|
| Synonyms |
GET-73; GET 73
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~363.28 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (9.08 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.08 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (9.08 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.6328 mL | 18.1640 mL | 36.3280 mL | |
| 5 mM | 0.7266 mL | 3.6328 mL | 7.2656 mL | |
| 10 mM | 0.3633 mL | 1.8164 mL | 3.6328 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
