Size | Price | Stock | Qty |
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500mg |
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1g |
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2g |
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5g |
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10g |
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Other Sizes |
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Purity: ≥98%
Targets |
Histamine H1 receptor; Histamine H2 receptor
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ln Vitro |
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ln Vivo |
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Cell Assay |
Histamine, bradykinin, and angiotensin II stimulate release of catecholamines from adrenal medulla. Here we show, using bovine adrenal chromaffin cells in culture, that these agonists as well as carbachol (with hexamethonium) stimulate production of inositol phosphates. The histamine response was mepyramine sensitive, implicating an H1 receptor, whereas bradykinin had a lower EC50 than Met-Lys-bradykinin, and [Des-Arg9]-bradykinin was relatively inactive, implicating a BK-2 receptor. Total inositol phosphates formed in the presence of lithium were measured, with histamine giving the largest response. The relative contribution of chromaffin cells and nonchromaffin cells in the responses was assessed. In each case chromaffin cells were found to be responding to the agonists; in the case of histamine the response was solely on chromaffin cells. When the inositol phosphates accumulating over 2 or 5 min, with no lithium present, were separated on Dowex anion-exchange columns, bradykinin gave the greatest stimulation in the inositol trisphosphate fraction, whereas histamine gave a larger inositol monophosphate accumulation. On resolution of the isomers of stimulated inositol trisphosphate after 2 min of stimulation, the principal isomer present was inositol 1,3,4-trisphosphate in each case. Two hypotheses for the differential responses to histamine and bradykinin are discussed[1].
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Animal Protocol |
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References | |||
Additional Infomation |
Histamine phosphate is a phosphate salt that is the diphosphate salt of histamine. It has a role as a histamine agonist. It contains a histamine.
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Molecular Formula |
C5H15N3O8P2
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Molecular Weight |
307.14
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Exact Mass |
307.03
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Elemental Analysis |
C, 19.55; H, 4.92; N, 13.68; O, 41.67; P, 20.17
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CAS # |
51-74-1
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Related CAS # |
Histamine dihydrochloride; 56-92-8; Histamine; 51-45-6
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PubChem CID |
65513
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Appearance |
White to off-white solid powder
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Boiling Point |
887.3ºC at 760 mmHg
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Melting Point |
128-132 °C
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tPSA |
142.27
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SMILES |
C1=C(NC=N1)CCN.OP(=O)(O)O.OP(=O)(O)O
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InChi Key |
ZHIBQGJKHVBLJJ-UHFFFAOYSA-N
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InChi Code |
InChI=1S/C5H9N3.2H3O4P/c6-2-1-5-3-7-4-8-5;2*1-5(2,3)4/h3-4H,1-2,6H2,(H,7,8);2*(H3,1,2,3,4)
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Chemical Name |
2-(1H-imidazol-5-yl)ethanamine;phosphoric acid
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Synonyms |
Histamine acid phosphate; Histamine diphosphate; Histamine phosphate; Histamine biphosphate; Histamine dihydrogen phosphate; Histamine diphosphate; 51-74-1; Histamine acid phosphate; Histamine biphosphate; 2-(1H-imidazol-4-yl)ethanamine bis(phosphate); Histamine phosphate (1:2); Histamine dihydrogen phosphate;
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
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Solubility (In Vivo) |
Solubility in Formulation 1: 50 mg/mL (162.79 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
 (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.2558 mL | 16.2792 mL | 32.5584 mL | |
5 mM | 0.6512 mL | 3.2558 mL | 6.5117 mL | |
10 mM | 0.3256 mL | 1.6279 mL | 3.2558 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
NCT Number | Recruitment | interventions | Conditions | Sponsor/Collaborators | Start Date | Phases |
NCT05131555 | Active Recruiting |
Drug: Placebo: Placebo D+ exercise training Drug: H1 blockade: H1 receptor Dantagonist + exercise training |
Exercise Histamine |
University Ghent | August 16, 2021 | Not Applicable |
NCT00362999 | Active Recruiting |
N/A | Allergic Rhinitis | Children's Mercy Hospital Kansas City |
August 2006 | N/A |
NCT06154824 | Recruiting | Other: Histamine Other: Cowhage |
Histamine Cowhage |
Aalborg University | December 15, 2023 | Not Applicable |
NCT06081998 | Recruiting | Other: Histamine Other: Cowhage |
Histamine Cowhage |
Aalborg University | November 1, 2023 | Not Applicable |
NCT06081946 | Completed | Other: Histamine Other: Cowhage |
Histamine Cowhage |
Aalborg University | December 15, 2023 | Not Applicable |