| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
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| ln Vitro |
The MIC range of H. pylori isolates in the agar dilution assay ranged from 0.01-0.17 μM, or 0.002-0.032 μg/mL. The strains ARHp172 (MIC 0.004–0.016 μg/mL) and ARHp246 (MIC 0.008–0.032 μg/mL) that are resistant to clarithromycin can be effectively inhibited by HPi1 [1]. Although HPi1 is at least eighteen times more concentrated than the minimum inhibitory concentration of Helicobacter pylori, it exhibits modest effect against Bacteroidetes. A MIC of 0.3 μg/mL was shown to indicate more powerful action for Campylobacter jejuni [1]. It has been observed that HPi1 possesses favorable physicochemical and pharmacological characteristics, such as determined water solubility (19 μg/mL), 93% binding to human plasma protein, human liver microsomal stability (T1/2 of 1.3 hours), and passively permeable membrane Ability[1].
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|---|---|
| ln Vivo |
At doses of 25 or 50 mg/kg/day, treatment with HPi1 (6.25-50 mg/kg; oral gavage; once daily; for 3 days; female C57BL/6 mice) decreased colony counts below the detection limit [ 1].
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| Animal Protocol |
Animal/Disease Models: Adult specific pathogen-free female C57BL/6 mice (6-8 weeks old), fed Helicobacter pylori SS1 suspension [1]
Doses: 6.25 mg/kg, 12.5 mg/kg, 25 mg/kg, 50 mg/kg Doses: po (oral gavage); one time/day; for 3 days. Experimental Results: The colony count diminished below the detection limit. |
| References |
| Molecular Formula |
C8H8N4S
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|---|---|
| Molecular Weight |
192.24
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| Exact Mass |
192.047
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| CAS # |
13080-21-2
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| PubChem CID |
781248
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.55g/cm3
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| Boiling Point |
376.1ºC at 760 mmHg
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| Flash Point |
181.3ºC
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| Vapour Pressure |
7.41E-06mmHg at 25°C
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| Index of Refraction |
1.794
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| LogP |
1.977
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
13
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| Complexity |
251
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
TVYAHCDRRICQJM-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C8H8N4S/c9-12-7-8(13)11-6-4-2-1-3-5(6)10-7/h1-4H,9H2,(H,10,12)(H,11,13)
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| Chemical Name |
3-hydrazinyl-1H-quinoxaline-2-thione
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| Synonyms |
HPi 1; HPi-1; HPi1
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~62.5 mg/mL (~325.11 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (10.82 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.2018 mL | 26.0092 mL | 52.0183 mL | |
| 5 mM | 1.0404 mL | 5.2018 mL | 10.4037 mL | |
| 10 mM | 0.5202 mL | 2.6009 mL | 5.2018 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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