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1mg |
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5mg |
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Other Sizes |
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ln Vitro |
BAY 59-9435 greatly lowers the culture medium's elevated IL-6 induced by isoproterenol. BAY 59-9435 inhibits the expression of SphK1 produced by isoproterenol [3].
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Cell Assay |
Western Blot analysis[3]
Cell Types: 3T3-L1 cells. Tested Concentrations: 10μM. Incubation Duration: 1 hour. Experimental Results: BAY pretreatment completely abolished the induction of SphK1 expression by CL. |
References |
[1]. Ebdrup S, et al. Synthesis and structure-activity relationship for a novel class of potent and selective carbamoyl-triazole based inhibitors of hormone sensitive lipase. J Med Chem. 2004 Jan 15;47(2):400-10.
[2]. Elizabeth A Rondini, et al. Novel Pharmacological Probes Reveal ABHD5 as a Locus of Lipolysis Control in White and Brown AdipocytesJ Pharmacol Exp Ther. 2017 Dec;363(3):367-376. [3]. Wenliang Zhang, et al. Adipocyte lipolysis-stimulated interleukin-6 production requires sphingosine kinase 1 activity. J Biol Chem. 2014 Nov 14;289(46):32178-85. |
Molecular Formula |
C14H22N2O3
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Molecular Weight |
266.336083889008
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Exact Mass |
266.163
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CAS # |
654059-21-9
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PubChem CID |
10038557
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Appearance |
Typically exists as solid at room temperature
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Density |
1.1±0.1 g/cm3
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Boiling Point |
345.8±25.0 °C at 760 mmHg
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Flash Point |
162.9±23.2 °C
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Vapour Pressure |
0.0±0.8 mmHg at 25°C
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Index of Refraction |
1.522
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LogP |
2.42
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Hydrogen Bond Donor Count |
0
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Hydrogen Bond Acceptor Count |
3
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Rotatable Bond Count |
1
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Heavy Atom Count |
19
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Complexity |
428
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Defined Atom Stereocenter Count |
1
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SMILES |
O=C1C(C(C)C)=C(C)N(C(N2C[C@@H](C)CCC2)=O)O1
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InChi Key |
XWESKOHENXWEAX-JTQLQIEISA-N
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InChi Code |
InChI=1S/C14H22N2O3/c1-9(2)12-11(4)16(19-13(12)17)14(18)15-7-5-6-10(3)8-15/h9-10H,5-8H2,1-4H3/t10-/m0/s1
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Chemical Name |
3-methyl-2-[(3S)-3-methylpiperidine-1-carbonyl]-4-propan-2-yl-1,2-oxazol-5-one
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~250 mg/mL (~938.65 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (7.81 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (7.81 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (7.81 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.7546 mL | 18.7730 mL | 37.5460 mL | |
5 mM | 0.7509 mL | 3.7546 mL | 7.5092 mL | |
10 mM | 0.3755 mL | 1.8773 mL | 3.7546 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.