IKK-16 (IKK Inhibitor VII; IKK 16)

Alias: IKK 16; IKK Inhibitor VII; IKK-16; IKK16
Cat No.:V0750 Purity: ≥98%
IKK-16 (also known as IKK Inhibitor VII; IKK-16; IKK16) is a novel, potent and selective IκBα kinase (IKK) inhibitor with potential anti-inflammatory activity.
IKK-16 (IKK Inhibitor VII; IKK 16) Chemical Structure CAS No.: 873225-46-8
Product category: IκB IKK
This product is for research use only, not for human use. We do not sell to patients.
Size Price Stock Qty
1mg
5mg
10mg
25mg
50mg
100mg
250mg
Other Sizes

Other Forms of IKK-16 (IKK Inhibitor VII; IKK 16):

  • IKK-16 HCl
Official Supplier of:
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Top Publications Citing lnvivochem Products
Purity & Quality Control Documentation

Purity: ≥98%

Product Description

IKK-16 (also known as IKK Inhibitor VII; IKK-16; IKK16) is a novel, potent, and selective IκBα inhibitor that may have anti-inflammatory effects. In cell-free assays, it inhibits IKK-2, IKK complex, and IKK-1 with IC50 values of 40 nM, 70 nM, and 200 nM, respectively. IKK-16 has also been discovered to be active in a mouse model of thioglycollate-induced peritonitis. Additionally, studies on rats and mice have shown that IKK-16 has significant in vivo activity in a model of acute cytokine release and is orally bioavailable in both species.

Biological Activity I Assay Protocols (From Reference)
Targets
IKK2 (IC50 = 40 nM); IKK1 (IC50 = 200 nM); IKK (IC50 = 70 nM); LRRK2 (IC50 = 50 nM)
ln Vitro
In HUVEC cells, IKK-16 prevents TNFα from triggering the expression of the adhesion molecules E-selectin, ICAM-1, and VCAM-1. Despite exhibiting activity in assays for IFNγ-induced expression of HLA-DR or β2 microglobulin, IKK-16's potency in these tests is 4- to 10-fold lower. [1]
ln Vivo
IKK-16 inhibits neutrophil extravasion in thioglycollate-induced peritonitis and LPS-induced TNF-α release in vivo. It is orally bioavailable in rats and mice.[1]
Enzyme Assay
IKK 16 is a selective IκB kinase (IKK) inhibitor for IKK2, IKK complex and IKK1 with IC50s of 40 nM, 70 nM and 200 nM, respectively. IKK16 also inhibits leucine-rich repeat kinase-2 (LRRK2) with an IC50 of 50 nM.
Cell Assay
SH-SY5Y cells are transduced with 25% (v/v) BacMam LRRK2-GFP G2019S and then plated (20 µL/well, 20,000 cells/well) onto eight 384-well assay plates. Then, for 90 min, 25% BacMam LRRK2-GFP G2019S transduced SH-SY5Y cells are incubated with the corresponding concentrations of the corresponding substances (e.g., IKK 16, 0.01, 0.1, 1, 10 and 100 μM), followed by the TR-FRET detection with Tb-anti-LRRK2 pSer935 antibody. Calculated is the inhibition percentage.
Animal Protocol
Rats and Mice: In two animal models, IKK 16 is tested. Its ability to prevent TNF from being released into the bloodstream after LPS challenge in rats is tested first. One hour before the LPS challenge, IKK 16 is dosed subcutaneously (sc) or orally (o) at a dose of 30 mg/kg. Plasma is collected four hours after the challenge, and a commercially available ELISA kit is used to assess the systemic TNFα levels. IKK 16 exhibits a notable 86% (sc) and 75% (p.o.) inhibition when administered via either route and at the recommended dose. IKK 16 is also active in the mouse model of peritonitis caused by thioglycollate in a subsequent experiment. In this model, a dose of 10 mg/kg sc results in an approximately 50% inhibition of neutrophil extravasation.
Mice: LPS (9 mg/kg body weight) and PepG (3 mg/kg body weight) are administered intraperitoneally to two-month-old male C57BL/6 mice. Sham mice receive the same care but are not exposed to LPS or PepG. Mice are given either IKK 16 (1 mg/kg body weight intravenously) or vehicle (5 mL/kg body weight 10% DMSO intravenously) at one hour after LPS/PepG co-administration. After 24 hours, the experiment is over, and blood and organ samples are taken in order to measure any organ dysfunction or injury. Four groups of mice are randomly assigned: (1) sham+vehicle (n=10); (2) sham+IKK 16 (n=3); (3) LPS/PepG+vehicle (n=9); (4) LPS/PepG+IKK 16 (n=10).
References

[1]. Bioorg Med Chem Lett . 2006 Jan 1;16(1):108-12.

These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C28H29N5OS
Molecular Weight
483.63
Exact Mass
483.21
Elemental Analysis
C, 69.54; H, 6.04; N, 14.48; O, 3.31; S, 6.63
CAS #
873225-46-8
Appearance
Solid powder
SMILES
C1CCN(C1)C2CCN(CC2)C(=O)C3=CC=C(C=C3)NC4=NC=CC(=N4)C5=CC6=CC=CC=C6S5
InChi Key
BWZJBXAPRCVCKQ-UHFFFAOYSA-N
InChi Code
InChI=1S/C28H29N5OS/c34-27(33-17-12-23(13-18-33)32-15-3-4-16-32)20-7-9-22(10-8-20)30-28-29-14-11-24(31-28)26-19-21-5-1-2-6-25(21)35-26/h1-2,5-11,14,19,23H,3-4,12-13,15-18H2,(H,29,30,31)
Chemical Name
[4-[[4-(1-benzothiophen-2-yl)pyrimidin-2-yl]amino]phenyl]-(4-pyrrolidin-1-ylpiperidin-1-yl)methanone
Synonyms
IKK 16; IKK Inhibitor VII; IKK-16; IKK16
HS Tariff Code
2934.99.9001
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO: ~97 mg/mL (~200.6 mM)
Water: <1 mg/mL
Ethanol: <1 mg/mL
Solubility (In Vivo)
30% PEG400+0.5% Tween80+5% propylene glycol: 30 mg/mL
 (Please use freshly prepared in vivo formulations for optimal results.)
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 2.0677 mL 10.3385 mL 20.6770 mL
5 mM 0.4135 mL 2.0677 mL 4.1354 mL
10 mM 0.2068 mL 1.0338 mL 2.0677 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

Calculator

Molarity Calculator allows you to calculate the mass, volume, and/or concentration required for a solution, as detailed below:

  • Calculate the Mass of a compound required to prepare a solution of known volume and concentration
  • Calculate the Volume of solution required to dissolve a compound of known mass to a desired concentration
  • Calculate the Concentration of a solution resulting from a known mass of compound in a specific volume
An example of molarity calculation using the molarity calculator is shown below:
What is the mass of compound required to make a 10 mM stock solution in 5 ml of DMSO given that the molecular weight of the compound is 350.26 g/mol?
  • Enter 350.26 in the Molecular Weight (MW) box
  • Enter 10 in the Concentration box and choose the correct unit (mM)
  • Enter 5 in the Volume box and choose the correct unit (mL)
  • Click the “Calculate” button
  • The answer of 17.513 mg appears in the Mass box. In a similar way, you may calculate the volume and concentration.

Dilution Calculator allows you to calculate how to dilute a stock solution of known concentrations. For example, you may Enter C1, C2 & V2 to calculate V1, as detailed below:

What volume of a given 10 mM stock solution is required to make 25 ml of a 25 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=25 μM, V2=25 ml and V1 is the unknown:
  • Enter 10 into the Concentration (Start) box and choose the correct unit (mM)
  • Enter 25 into the Concentration (End) box and select the correct unit (mM)
  • Enter 25 into the Volume (End) box and choose the correct unit (mL)
  • Click the “Calculate” button
  • The answer of 62.5 μL (0.1 ml) appears in the Volume (Start) box
g/mol

Molecular Weight Calculator allows you to calculate the molar mass and elemental composition of a compound, as detailed below:

Note: Chemical formula is case sensitive: C12H18N3O4  c12h18n3o4
Instructions to calculate molar mass (molecular weight) of a chemical compound:
  • To calculate molar mass of a chemical compound, please enter the chemical/molecular formula and click the “Calculate’ button.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
  • Molecular mass (or molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
  • Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
/

Reconstitution Calculator allows you to calculate the volume of solvent required to reconstitute your vial.

  • Enter the mass of the reagent and the desired reconstitution concentration as well as the correct units
  • Click the “Calculate” button
  • The answer appears in the Volume (to add to vial) box
In vivo Formulation Calculator (Clear solution)
Step 1: Enter information below (Recommended: An additional animal to make allowance for loss during the experiment)
Step 2: Enter in vivo formulation (This is only a calculator, not the exact formulation for a specific product. Please contact us first if there is no in vivo formulation in the solubility section.)
+
+
+

Calculation results

Working concentration mg/mL;

Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
             (2) Be sure to add the solvent(s) in order.

Biological Data
Contact Us