| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| Other Sizes |
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| ln Vitro |
Imipramine (0.5-300 μM, 3 days) reduces the viability of HCT-116 cells [1]. Cell invasion (48 hours) and migration (7.5 hours) are inhibited by imipramine (20 μM) [1]. In U-87MG glioma cells, imipramine (50 μM, 0-240 min) suppresses the PI3K/Akt/mTOR signaling pathway [2]. In U-87MG glioma cells, imipramine (60 μM, 24 hours) promotes autophagy [2]. In HL-60 cells, imipramine (80 μM, 24 hours) causes apoptosis [3].
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| ln Vivo |
Imipramine (20 mg/kg intraperitoneally or 15 mg/kg orally; given daily for 24 days) attenuates neuroinflammatory signaling and reverses stress-induced social avoidance in mice [4].
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| Cell Assay |
Cell Viability Assay[1]
Cell Types: DLD-1, HCT-116 and SW-480 Tested Concentrations: 0.5-300 μM Incubation Duration: 3 days Experimental Results: Inhibition of cell viability, HCT-116 is more sensitive than DLD-1 and SW-480 . Cell migration assay[1] Cell Types: SW-480, DLD-1 and HCT-116 Tested Concentrations: 20 μM Incubation Duration: 7 hrs (hours) Experimental Results: Significant migration inhibition was produced in all cell lines tested. Cell invasion experiment [1] Cell Types: HCT-116 Tested Concentrations: 20 μM Incubation Duration: 48 h Experimental Results: Matrigel inhibits cell invasion. Western Blot Analysis[2] Cell Types: U-87MG Tested Concentrations: 50 μM Incubation Duration: 0, 15, 30, 60, 120 and 240 minutes Experimental Results: Significant time-dependent inhibition of phosphorylation of Akt (Ser473) and mTOR (Ser2481) The way. Also dephosphorylates p70 S6K, a downstream target of mTOR. Autophagy assay [2] Cell Types: U-87MG Tested Concentrations: 60 μM Incubation Duration: 24 hrs (hours) Experimental Results: Induction of autophagy is stimulated by redistribution of LC3 in U-87MG glioma cells. Autophagy d |
| Animal Protocol |
Animal/Disease Models: Male C57BL/6 mice (6-8 weeks old) were subjected to RSD (repeated social defeat) and HCC (home cage control) [4]
Doses: 20 mg/kg or 15 mg/kg Route of Administration: intraperitoneal (ip) injection or administered orally daily for 24 days. Experimental Results: Reversed RSD-induced social avoidance behavior, Dramatically increased interaction time, and Dramatically diminished stress-induced IL-6 mRNA levels in brain microglia. |
| References |
[1]. Alburquerque-González B, et al. New role of the antidepressant imipramine as a Fascin1 inhibitor in colorectal cancer cells. Exp Mol Med. 2020 Feb;52(2):281-292.
[2]. Jeon SH, et al. The tricyclic antidepressant imipramine induces autophagic cell death in U-87MG glioma cells. Biochem Biophys Res Commun. 2011 Sep 23;413(2):311-7. [3]. Xia Z, et al. The antidepressants imipramine, clomipramine, and citalopram induce apoptosis in human acute myeloid leukemia HL-60 cells via caspase-3 activation. J Biochem Mol Toxicol. 1999;13(6):338-47. [4]. Ramirez K, et al. Imipramine attenuates neuroinflammatory signaling and reverses stress-induced social avoidance. Brain Behav Immun. 2015 May;46:212-20. [5]. Balkovetz DF, et al. Evidence for an imipramine-sensitive serotonin transporter in human placental brush-border membranes. J Biol Chem. 1989 Feb 5;264(4):2195-8. |
| Molecular Formula |
C19H24N2
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|---|---|
| Molecular Weight |
280.4073
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| CAS # |
113-52-0
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| Related CAS # |
Imipramine-d4 hydrochloride;61361-33-9;Imipramine;50-49-7;Imipramine-d6;65100-45-0;Imipramine-d3 hydrochloride;112898-42-7
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| Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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| SMILES |
N1(C2=C([H])C([H])=C([H])C([H])=C2C([H])([H])C([H])([H])C2=C([H])C([H])=C([H])C([H])=C12)C([H])([H])C([H])([H])C([H])([H])N(C([H])([H])[H])C([H])([H])[H]
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 100 mg/mL (~315.59 mM)
H2O : ~62.5 mg/mL (~197.24 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (7.89 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (7.89 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (7.89 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 60 mg/mL (189.35 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.5662 mL | 17.8310 mL | 35.6621 mL | |
| 5 mM | 0.7132 mL | 3.5662 mL | 7.1324 mL | |
| 10 mM | 0.3566 mL | 1.7831 mL | 3.5662 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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