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100mg | ||
250mg | ||
500mg |
ln Vitro |
IR 813 (17.3 μM) induces red blood cell hemolysis at a rate of 31.4% [1]. In MRC-5 cells, IR 813 (5.9 μM) causes 50% cell death within 24 hours [1].
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ln Vivo |
IR 813 perchlorate demonstrated fast extravasation and peak fluorescence intensity four hours after injection when used to monitor regional lymph nodes in mice [1]. Advice (This is our suggested protocol, which should be adjusted to suit your particular requirements as it only offers guidance) [1]. 1. Animals: In order to decrease tissue autofluorescence in the NIR region, ten-week-old female Balb/cOlaAnN mice were given an unlimited supply of food and water and kept on a 12-hour light/dark cycle. 2. Dosage: The mouse was given a single subcutaneous injection in its right front paw of 5.1 nmol IR 813 dye (20 μL 0.173 mg/mL, dissolved in PEG-400/ethanol/water=3:2:5, v/v/v solution). ..3. For in vivo optical imaging, utilize the Fluobeam700 NIR imaging system (739 nm excitation light, 3.5 mW), with a 750 nm long-pass emission cutoff filter. A week's worth of data on the fluorescence intensity in axillary lymph nodes (ALN) was collected (5 minutes, 1 hour, 4 hours, 24 hours, and 7 days). 4. Data analysis: To extract semi-quantitative information from fluorescence images, use ImageJ 1.44 software.
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References |
[1]. Marion Hell, et al. Surface chemistry architecture of silica nanoparticles determine the efficiency of in vivo fluorescence lymph node mapping. ACS Nano. 2013 Oct 22;7(10):8645-57.
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Molecular Formula |
C40H40N2CL+.O4CL-
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Molecular Weight |
683.6626
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CAS # |
201024-57-9
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Related CAS # |
IR 813 tosylate;134127-48-3
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
O=Cl([O-])(=O)=O.ClC1/C(=C/C=C2/N(C)C3C=CC4=CC=CC=C4C=3C/2(C)C)/CCCC=1C=CC1=[N+](C)C2C=CC3=CC=CC=C3C=2C1(C)C |t:33|
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80: Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 :Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.4627 mL | 7.3136 mL | 14.6272 mL | |
5 mM | 0.2925 mL | 1.4627 mL | 2.9254 mL | |
10 mM | 0.1463 mL | 0.7314 mL | 1.4627 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.