| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| Other Sizes |
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| Targets |
iNOS
|
|---|---|
| ln Vitro |
LPS-stimulated RAW 264.7 macrophage viability is unaffected by isisin at doses up to 40 μM [1]. In RAW 264.7 macrophages, irisflorentin (10–40 μM) suppresses LPS-induced NO generation in a concentration-dependent manner without changing iNOS activity [1]. LPS (10 ng/mL)-stimulated RAW 264.7 macrophages exhibit a rise in iNOS mRNA expression that is concentration-dependently inhibited by irisflorentin (10–40 μM). The production of TNF-α, IL-1β, and IL-6 in LPS-stimulated RAW 264.7 macrophages is concentration-dependently inhibited by irisflorentin (10–40 μM) [1]. LPS (10 ng/mL)-induced elevation of iNOS protein levels is inhibited concentration-dependently by irisflorentin (10–40 μM). A concentration-dependent effect of irisflorentin (10–40 μM) suppresses LPS-induced phosphorylation of p38 and ERK1/2, while having minimal effect on JNK phosphorylation [1].
|
| Cell Assay |
RT-PCR[1]
Cell Types: LPS-stimulated RAW 264.7 macrophages Tested Concentrations: 10, 20, 40 μM Incubation Duration: Experimental Results: Inhibition of LPS (10 ng/mL)-induced increase in iNOS mRNA expression. Inhibits the production of TNF-α, IL-1β and IL-6. Western Blot Analysis [1] Cell Types: LPS-stimulated RAW 264.7 macrophages Tested Concentrations: 10, 20, 40 μM Incubation Duration: Experimental Results: Inhibition of LPS (10 ng/mL)-induced increase in iNOS protein levels. Inhibits LPS-induced phosphorylation of p38 and ERK1/2, but slightly affects JNK phosphorylation. |
| References | |
| Additional Infomation |
Irisflorentin is a member of 4'-methoxyisoflavones.
Irisflorentin has been reported in Iris tectorum, Iris potaninii, and other organisms with data available. |
| Molecular Formula |
C20H18O8
|
|---|---|
| Molecular Weight |
386.3521
|
| Exact Mass |
386.1
|
| CAS # |
41743-73-1
|
| PubChem CID |
170569
|
| Appearance |
White to off-white solid
|
| Density |
1.3±0.1 g/cm3
|
| Boiling Point |
569.1±50.0 °C at 760 mmHg
|
| Melting Point |
169 °C
|
| Flash Point |
250.5±30.2 °C
|
| Vapour Pressure |
0.0±1.6 mmHg at 25°C
|
| Index of Refraction |
1.593
|
| LogP |
3.19
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
8
|
| Rotatable Bond Count |
5
|
| Heavy Atom Count |
28
|
| Complexity |
593
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
O1C([H])=C(C2C([H])=C(C(=C(C=2[H])OC([H])([H])[H])OC([H])([H])[H])OC([H])([H])[H])C(C2=C(C3=C(C([H])=C12)OC([H])([H])O3)OC([H])([H])[H])=O
|
| InChi Key |
RISXUTCDCPHJFQ-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C20H18O8/c1-22-13-5-10(6-14(23-2)18(13)24-3)11-8-26-12-7-15-19(28-9-27-15)20(25-4)16(12)17(11)21/h5-8H,9H2,1-4H3
|
| Chemical Name |
9-methoxy-7-(3,4,5-trimethoxyphenyl)-[1,3]dioxolo[4,5-g]chromen-8-one
|
| Synonyms |
Irisflorentin
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~129.42 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.17 mg/mL (5.62 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 21.7 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.5883 mL | 12.9416 mL | 25.8833 mL | |
| 5 mM | 0.5177 mL | 2.5883 mL | 5.1767 mL | |
| 10 mM | 0.2588 mL | 1.2942 mL | 2.5883 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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