3-Methylbutanamide; NPS-1776;Isovaleramide; NPS 1776; NPS1776;
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100mg |
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250mg |
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500mg |
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1g |
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Other Sizes |
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Isovaleramide (NPS-1776; NPS 1776; NPS1776; 3-Methylbutanamide), a natural product found in Valeriana pavonii, is a potent anticonvulsant agent that acts as an inhibitor of alcohol dehydrogenases. Isovaleramide (300 µM) exhibits a 42% of inhibition of the binding of ³H-FNZ to its sites in the in vitro assay. Isovaleramide showed no effect at concentrations up to 1000 μM in a variety of in vitro neurotransmitter binding or uptake assays, suggesting that its action does not involve a direct receptor-mediated effect at those systems studied.
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ln Vivo |
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Animal Protocol |
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References |
Biomedica.2010 Apr-Jun;30(2):245-50;Epilepsy Res.2004 Sep-Oct;61(1-3):1-48.
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Molecular Formula |
C5H11NO
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Molecular Weight |
101.15
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CAS # |
541-46-8
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Related CAS # |
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
O=C(C([H])([H])C([H])(C([H])([H])[H])C([H])([H])[H])N([H])[H]
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InChi Key |
SANOUVWGPVYVAV-UHFFFAOYSA-N
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InChi Code |
InChI=1S/C5H11NO/c1-4(2)3-5(6)7/h4H,3H2,1-2H3,(H2,6,7)
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Chemical Name |
3-Methylbutanamide
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Synonyms |
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (20.56 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (20.56 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 9.8863 mL | 49.4315 mL | 98.8631 mL | |
5 mM | 1.9773 mL | 9.8863 mL | 19.7726 mL | |
10 mM | 0.9886 mL | 4.9432 mL | 9.8863 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.