| Size | Price | Stock | Qty |
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| 10mg |
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| 25mg |
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| 50mg |
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ITE is a novel and potent agonist of aryl hydrocarbon receptor (AhR) with immunosuppressive activity. It activates AHR with a Ki of 3 nM.
| ln Vitro |
ITE, an endogenous agonist of AhR, binds to AHR directly at a concentration of 3 nM [1]. There is a T cell proliferation response when ITE (0.03-30 mg/mL) decreases [2]. At 10 and 20 μM, ITE efficiently inhibits the development of human pulmonary artery endothelial cells (HPAEC), whereas at 0.01–5 μM, it has no effect. ITE had no effect on HPAECs' cell process cycle at 10 or 20 μM, and at 20 μM, it did not cause caspase to be induced in HPAEC hubs. Additionally, in HPAECs, ITE (20 μM) decreased AhR protein levels and raised CYP1A1 and CYP1B1 mRNA levels [3].
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| ln Vivo |
In mice, ITE (200 μg, ip) dramatically prevents the onset of experimental autoimmune uveitis (EAU). In mice, ITE decreased the percentage of cells producing IL-17, IL-10, or IFN-γ. Moreover, ITE reduced the small cell ratio of inflammatory cytokines in mouse LN cells [2].
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| References |
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| Additional Infomation |
2-[1H-indol-3-yl(oxo)methyl]-4-thiazolecarboxylic acid methyl ester is an indolyl carboxylic acid.
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| Molecular Formula |
C14H10N2O3S
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|---|---|
| Molecular Weight |
286.3058
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| Exact Mass |
286.041
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| CAS # |
448906-42-1
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| PubChem CID |
4668801
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.427g/cm3
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| Boiling Point |
520.4ºC at 760 mmHg
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| Flash Point |
268.5ºC
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| Index of Refraction |
1.688
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| LogP |
2.642
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
20
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| Complexity |
404
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
KDDXOGDIPZSCTM-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C14H10N2O3S/c1-19-14(18)11-7-20-13(16-11)12(17)9-6-15-10-5-3-2-4-8(9)10/h2-7,15H,1H3
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| Chemical Name |
methyl 2-(1H-indole-3-carbonyl)-1,3-thiazole-4-carboxylate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 41 mg/mL (~143.20 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.67 mg/mL (9.33 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 26.7 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. Solubility in Formulation 2: 2.08 mg/mL (7.26 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. View More
Solubility in Formulation 3: 10 mg/mL (34.93 mM) in 17% Polyethylene glycol 12-hydroxystearate in Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.4927 mL | 17.4636 mL | 34.9272 mL | |
| 5 mM | 0.6985 mL | 3.4927 mL | 6.9854 mL | |
| 10 mM | 0.3493 mL | 1.7464 mL | 3.4927 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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