| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| Other Sizes |
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| ln Vitro |
IXA6 (10 µM; 4 or 18 hours) preferentially triggers the transcriptional response of XBP1 and stimulates the IRE1-XBP1 communication [1]. IXA6 (10 µM; 4 hours) exhibits selectivity for the modification of endoplasmic reticulum proteostasis that is dependent on IRE1-XBP1s [1]. IXA6 (10 µM; 18 hours) inhibits APP secretion by activating IRE1 [1].
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|---|---|
| Cell Assay |
Western Blot Analysis[1]
Cell Types: HEK293T Cell Tested Concentrations: 10 µM Incubation Duration: 18 hrs (hours) Experimental Results: Increase in ER proteostasis factor gene expression corresponds to an increase in protein levels. RT-PCR[1] Cell Types: HEK293T Cell Tested Concentrations: 10 µM Incubation Duration: 4 hrs (hours) Experimental Results: Genome activation of IRE1-XBP1s to approximately 30-40% of Tg levels observed (Tg represents 100% activation of each gene). Cell viability assay[1] Cell Types: HEK293T Cell Tested Concentrations: 10 µM Incubation Duration: 4 hrs (hours) Experimental Results: A high level of overlap (64%) in genes induced by XBP1s and IXA6 was observed. RT-PCR[1] Cell Types: Huh7 and SHSY5Y cells Tested Concentrations: 10 µM Incubation Duration: 4 hrs (hours) Experimental Results: XBP1s mRNA was selectively upregulated in cell lines including Huh7 and SHSY5Y cells. RT-PCR[1] Cell Types: CHO cells Tested Concentrations: 10 µM Incubation Duration: 18 hrs (hours) Experimental Results: Co-treatment with 4µ8c was observed to block the reduction in Aβ secretion, confirming that this reduction is dependent on stable e |
| References |
| Molecular Formula |
C22H20CLN3O3S
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|---|---|
| Molecular Weight |
441.930502891541
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| Exact Mass |
441.091
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| CAS # |
1021106-40-0
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| PubChem CID |
42111339
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| Appearance |
White to light yellow solid powder
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| LogP |
3.2
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
30
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| Complexity |
690
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| Defined Atom Stereocenter Count |
0
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| SMILES |
ClC1C=CC(=CC=1)CN(CC(N1C2C=CC=CC=2CC1)=O)S(C1C=NC=CC=1)(=O)=O
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| InChi Key |
INLQYLVXKDCKIT-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C22H20ClN3O3S/c23-19-9-7-17(8-10-19)15-25(30(28,29)20-5-3-12-24-14-20)16-22(27)26-13-11-18-4-1-2-6-21(18)26/h1-10,12,14H,11,13,15-16H2
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| Chemical Name |
N-[(4-chlorophenyl)methyl]-N-[2-(2,3-dihydroindol-1-yl)-2-oxoethyl]pyridine-3-sulfonamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~33.33 mg/mL (~75.42 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (2.83 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.2628 mL | 11.3140 mL | 22.6280 mL | |
| 5 mM | 0.4526 mL | 2.2628 mL | 4.5256 mL | |
| 10 mM | 0.2263 mL | 1.1314 mL | 2.2628 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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